Role of the extracellular matrix in morphogenesis.
ABSTRACT The extracellular matrix is a complex, dynamic and critical component of all tissues. It functions as a scaffold for tissue morphogenesis, provides cues for cell proliferation and differentiation, promotes the maintenance of differentiated tissues and enhances the repair response after injury. Various amounts and types of collagens, adhesion molecules, proteoglycans, growth factors and cytokines or chemokines are present in the tissue- and temporal-specific extracellular matrices. Tissue morphogenesis is mediated by multiple extracellular matrix components and by multiple active sites on some of these components. Biologically active extracellular matrix components may have use in tissue repair, regeneration and engineering, and in programming stem cells for tissue replacement.
- SourceAvailable from: PubMed Central[Show abstract] [Hide abstract]
ABSTRACT: Currently available injectable fillers have demonstrated limited durability. This report proposes the in vitro culture of human adipose-derived stem cells (hASCs) on hyaluronic acid (HA) gel for in vivo growth of de novo adipose tissue. For in vitro studies, hASCs were isolated from human adipose tissue and were confirmed by multi-lineage differentiation and flow cytometry. hASCs were cultured on HA gel. The effectiveness of cell attachment and proliferation on HA gel was surveyed by inverted light microscopy. For in vivo studies, HA gel containing hASCs, hASCs without HA gel, HA gel alone were allocated and subcutaneously injected into the subcutaneous pocket in the back of nude mice (n=6) in each group. At eight weeks post-injection, the implants were harvested for histological examination by hematoxylin and eosin (H&E) stain, Oil-Red O stain and immunohistochemical staining. The human-specific Alu gene was examined. hASCs were well attachment and proliferation on the HA gel. In vivo grafts showed well-organized new adipose tissue on the HA gel by histologic examination and Oil-Red O stain. Analysis of neo-adipose tissues by PCR revealed the presence of the Alu gene. This study demonstrated not only the successful culture of hASCs on HA gel, but also their full proliferation and differentiation into adipose tissue. The efficacy of injected filler could be permanent since the reduction of the volume of the HA gel after bioabsorption could be replaced by new adipose tissue generated by hASCs. This is a promising approach for developing long lasting soft tissue filler.International journal of medical sciences. 01/2015; 12(2):154-62.
- [Show abstract] [Hide abstract]
ABSTRACT: We have developed a bilayered dermal-epidermal scaffold for application in the treatment of full thickness skin defects. The dermal component gels in situ and adapts to the lesion shape, delivering human dermal fibroblasts in a matrix of fibrin and cross-linked hyaluronic acid modified with a cell adhesion-promoting peptide. Fibroblasts were able to form a tridimensional matrix due to material features such as tailored mechanical properties, presence of protease degradable elements and cell binding ligands. The epidermal component is a robust membrane containing cross-linked hyaluronic acid and poly-L-lysine, on which keratinocytes were able to attach and to form a monolayer. Amine-aldehyde bonding at the interface between the two components allows the formation of a tightly bound composite scaffold. Both parts of the scaffold were designed to provide cell type specific cues to allow for cell proliferation and form a construct that mimics the skin environment.Acta Biomaterialia 12/2014; · 5.68 Impact Factor
- [Show abstract] [Hide abstract]
ABSTRACT: With the development of modern medical technology, the clinical range of biomaterial applications have gradually expanded. However, bioactivity on the surface of biomaterials may be reduced and induce thrombus formation that will not only cause serious dysfunction of the biological materials, but can also affect the host. Modification of these biological materials can thus help to retain the biological activity on the surface of the material and inhibit thrombus formation, which underlines the importance of their clinical application. The current review will address the modification of biomaterials and inhibition of thrombosis on the surface of biomaterials.Advanced Materials Research. 12/2013; 873:635-641.