[Stability and antioxidant activity of black currant and black aronia berry juices].
ABSTRACT The berries of black currant and black aronia are rich in polyphenolic compounds and especially in anthocyanins, demonstrating antioxidant activity. The aim of the study was to evaluate the possible effect of thermal technological processes on the quantity of polyphenols and anthocyanins in berry juice concentrates, and on the antioxidant activity. After 8 hour storage of black currant and black aronia berry juice concentrates at 60 degrees C, the amount of polyphenols decreased by 46% and 22%, anthocyanins 31% and 35%, respectively. Antioxidant activity decreased by 26% and 56%, respectively. The results demonstrated insufficient stability of juice concentrates, and impropriety of application of long lasting drying processes in manufacturing of black currant and black aronia berry dry products. Fast and efficient drying methods for liquid products should be applied to preserve qualitative and quantitative composition and their antioxidant activity.
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ABSTRACT: Products derived from the black chokeberry, Aronia melanocarpa, are claimed to be beneficial in disorders or diseases associated with oxidative stress. The claims are based on evidence from in vitro studies and animal experiments. The active principle--a mixture of procyanidins, anthocyanins and phenolic acids--constitutes one of the most potent natural antioxidants. A systematic review was carried out of the quality of the clinical trials on chokeberry products that had been published up to December 2009, and conventionally established criteria were used to assess the strength of the evidence for their clinical effectiveness. Thirteen studies were identified. The quality of most of the trials and, correspondingly, the evidence of effectiveness for Aronia products is poor. Though laboratory and clinical data indicate that chokeberry products may well be useful as 'functional food' for disorders or diseases related to oxidative stress, these promising indications need to be confirmed in more rigorous studies before putative therapeutic uses can be confidently recommended for chokeberry products.Phytotherapy Research 01/2010; 24(8):1107-14. DOI:10.1002/ptr.3226 · 2.40 Impact Factor
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ABSTRACT: Vaccinium uliginosum L. is a type of blueberry found in the Chinese Changbai Mountains. We extracted Vaccinium uliginosum Anthocyanins (A(V.uli)) to investigate its bioactivity on suppressing cancer cells. A(V.uli) was extracted under different conditions of temperature (10°C - 35°C), pH 1.0 - 3.0, and diatomaceous earth (1.0 g - 3.0 g), followed by a HPLC analysis for the determination of the ingredients. Its anticancer bioactivities on human colon and colorectal cancer cells (DLD-1 and COLO205) were compared with those on Lonicera caerulea Anthocyanins (A(L.cae)) and Vaccinium myrtillus Anthocyanins (A(V.myr)), using cell viability assays, DNA electrophoresis and nuclear morphology assays. The optimum process of A(V.uli) extraction involved conditions of temperature 20°C, pH 2.0, and diatomaceous earth 1.0 g/50 g of fruit weight. A(V.uli) contained 5 main components: delphinidin (40.70 ± 1.72)%, cyanidin (3.40 ± 0.68)%, petunidin (17.70 ± 0.54)%, peonidin (2.90 ± 0.63)% and malvidin (35.50 ± 1.11)%. The malvidin percentage was significantly higher (P < 0.05) than it in A(V.myr). A(V.uli) complied with a dose-dependent repression of cancer cell proliferation with an IC(50) (50% inhibitory concentration) value of 50 µg/ml, and showed greater anticancer efficiency than A(L.cae) and A(V.myr) under the same cell treatment conditions. These observations were further supported by the results of nuclear assays. The extraction protocol and conditions we used were effective for anthocyanin extraction. A(V.uli) could be a feasible practical research tool and a promising therapeutic source to suppress human colon or colorectal cancers.Chinese medical journal 10/2010; 123(19):2714-9. · 1.02 Impact Factor
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ABSTRACT: The changes in the stability of antioxidant capacity with time and its relation to the phenolic content were evaluated in eight Indian herbal teas. These herbal teas are claimed to be antistress, immunomodulator, anti-aging, anti-inflammatory, appetizer, stimulant, blood purifier, energizer, antidepressant, antidiabetic, rejuvenative, analgesic, antiviral, nervine, diuretic, antihypertensive and memory enhancer. Antioxidant capacity was determined over a period of 15 months from the date of their procurement using assays for SOD mimetic activity, LPO inhibitory capacity and total thiol content, which decreased positively with time. Total phenolic content was determined spectrometrically according to the Folin-Ciocalteu method and calculated as gallic acid equivalents (GAE). Herbal teas with higher phenolic content showed a comparatively less decline in antioxidant capacity. The SOD mimetic activity values in control samples (at the time of procurement) were seen to be in the range of 54.63–93.64 units/min/mg of extract which after 15 months of storage decreased upto 7.4-folds in some samples. LPO inhibitory capacity was observed upto 96.75% in herbal tea E at the time of procurement which dropped to 63.85% inhibition of MDA formation/5μl of extract after 15 months. In case of total thiol, the values were seen in the range of 0.55–1.71mg/g and after 15 months it was from 0.12 to 0.21mg/g. In all these cases high antioxidant activity was seen in the samples with higher phenolic content which also showed comparatively less decline in antioxidant capacity after considerable storage time. The results have significance, as most of the herbal teas available in the local markets in India do not carry any information regarding the period of use without decline in its beneficial effects.Food Research International 03/2006; 39(2):176-181. DOI:10.1016/j.foodres.2005.07.004 · 3.05 Impact Factor