Gene delivery using physical methods: an overview

Department of Medicine, University of Massachusetts Medical School, Worcester, MA, USA.
Methods in Molecular Biology (Impact Factor: 1.29). 02/2004; 245:147-66.
Source: PubMed
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    • "The method can be adapted to a wide range of applications and is used extensively. Its main disadvantages are the lack of quantitative delivery, cell toxicity, damage to sensitive delivery materials and limited success in protein delivery [8] [9]. Sonoporation has been proposed as an alternative to electroporation [5] [6]. "
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    ABSTRACT: We present a microfluidic based injection system designed to achieve intracellular delivery of macromolecules by directing a picoliter-jet of a solution towards individual cells. After discussing the concept, we present design specification and criteria, elucidate performance and discuss results. The method has the potential to be quantitative and high throughput, overcoming limitations of current intracellular delivery protocols.
    Journal of Micromechanics and Microengineering 03/2013; 23(3). DOI:10.1088/0960-1317/23/3/035026 · 1.73 Impact Factor
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    • "In this protocol, microscopic tungsten spheres are used to deliver DNA or RNA constructs. This method has been used to transfect plants cells, muscle cells, and various cultured cells including epithelial cells, endothelial cells, and monocytes (Chou et al., 2004). Gene delivery using electroporation is the most versatile method and can be applied to a wide variety of cell types. "
    Embryonic Stem Cells - Basic Biology to Bioengineering, 09/2011; , ISBN: 978-953-307-278-4
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