Gene delivery using physical methods: an overview

Department of Medicine, University of Massachusetts Medical School, Worcester, MA, USA.
Methods in Molecular Biology (Impact Factor: 1.29). 02/2004; 245:147-66.
Source: PubMed
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    • "Encryption embeds a particular message or information within a DNA sequence. In labeling, the DNA sequence containing the encrypted information is assimilated into a target organism by genetic engineering (Chou et al. 2004; Mercenier and Chassy 1988; Newell 2000). "
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    ABSTRACT: We established a DNA watermarking system for discriminating transgenic plants. The system contains an encryption algorithm based on a binary system, genetic transformation and a detection algorithm for encrypted DNA watermark sequences using a DNA dot plot. The encryption algorithm converted character strings into nucleic acid sequences through binary digits, and the sequence was designed to be resistant to transition mutations to decipher codes completely. Moreover, the encrypted sequences were capable of taking specific nucleotide sequences in using the algorithmic redundancy of the corresponding DNA. Genetic transformation enables labeling plant genomes with DNA watermarks. The detection algorithm allows finding traces of sequence changes in DNA watermarks, complementing the error protection function of the encryption algorithm. To validate the effectiveness of our DNA watermarking system, we introduced a DNA watermark to the tobacco genome and detected the DNA watermark in PCR products amplified from the genome. This indicates that DNA watermark technology is useful for introducing artificial genetic markers in plant organisms, in particular when several transgenic host plants and transgenes are used. The source codes of the Perl scripts are available in this report.
    Plant Biotechnology 01/2014; 31(3):241-248. DOI:10.5511/plantbiotechnology.14.0609b · 0.87 Impact Factor
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    • "The method can be adapted to a wide range of applications and is used extensively. Its main disadvantages are the lack of quantitative delivery, cell toxicity, damage to sensitive delivery materials and limited success in protein delivery [8] [9]. Sonoporation has been proposed as an alternative to electroporation [5] [6]. "
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    ABSTRACT: We present a microfluidic based injection system designed to achieve intracellular delivery of macromolecules by directing a picoliter-jet of a solution towards individual cells. After discussing the concept, we present design specification and criteria, elucidate performance and discuss results. The method has the potential to be quantitative and high throughput, overcoming limitations of current intracellular delivery protocols.
    Journal of Micromechanics and Microengineering 03/2013; 23(3). DOI:10.1088/0960-1317/23/3/035026 · 1.73 Impact Factor
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    • "In this protocol, microscopic tungsten spheres are used to deliver DNA or RNA constructs. This method has been used to transfect plants cells, muscle cells, and various cultured cells including epithelial cells, endothelial cells, and monocytes (Chou et al., 2004). Gene delivery using electroporation is the most versatile method and can be applied to a wide variety of cell types. "

    Embryonic Stem Cells - Basic Biology to Bioengineering, 09/2011; , ISBN: 978-953-307-278-4
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