Article

[Utilization of polymerase chain reaction for detection and genotyping of Helicobacter pylori in gastric biopsy using paraffin: a retrospective study].

Centro de Investigaciones en Genética Básica y Aplicada (CIGEBA), Fac. de Cs. Veterinarias-UNLP, Calle 60 y 118 s/n B1900VW, La Plata, Argentina.
Acta gastroenterologica Latinoamericana 02/2003; 33(4):193-8. pp.193-8
Source: PubMed

ABSTRACT Helicobacter pylori infection is presumed to be the major causal agent of chronic active gastritis in humans. The persistent infection with this pathogen would be an important factor in the pathogenesis of peptic ulcer and also gastric cancer.
We investigated relationship between H. pylori characteristics in 42 patients with normal mucosa or gastritis with minor changes and 40 patients with mild and severe gastritis. Detection and typing of vacA and cagA genes were performed using a polymerase chain reaction method.
The analysis of vacA prevalence and the type (S1 or S2) showed non-significant differences between the two groups studied (p > 0.05). However, cagA analysis showed highly significant differences between the groups classified as normal tissue-weak gastritis and mild-severe gastritis (p < 0.0001; OR = 8.4; CI = 3.1-22.8).
cagA status is associated to the grade of gastritis, finding higher frequencies of H. pylori cagA+ in the moderate-severe gastritis group. These highly significant differences could make cagA status a genetic marker for disease progress.

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Keywords

40 patients
 
42 patients
 
cagA genes
 
chronic active gastritis
 
disease progress
 
gastric cancer
 
H. pylori cagA+
 
H. pylori characteristics
 
Helicobacter pylori infection
 
higher frequencies
 
major causal agent
 
mild-severe gastritis
 
minor changes
 
moderate-severe gastritis group
 
non-significant differences
 
normal tissue-weak gastritis
 
persistent infection
 
polymerase chain reaction method
 
severe gastritis
 
vacA prevalence