Article

The lateral line of zebrafish: a model system for the analysis of morphogenesis and neural development in vertebrates.

Laboratoire de Neurogénétique, INSERM E343 Université Montpellier II, cc103 Place E. Bataillon, 34095 Montpellier, France.
Biology of the Cell (Impact Factor: 3.87). 01/2004; 95(9):579-87. DOI: 10.1016/j.biolcel.2003.10.005
Source: PubMed

ABSTRACT The lateral line of the zebrafish has many of the advantages that made the sensory organs of Drosophila a very productive model system: 1) it comprises a set of discrete sense organs (neuromasts) arranged in a defined, species-specific pattern, such that each organ can be individually recognized; 2) the neuromasts are superficial and easy to visualize, and the innervating neurons are easy to label; 3) the sensory projection is simple yet reproducibly organized. Here we describe some of the tools that can be used to investigate the development of this system, and we illustrate their usefulness with specific examples. We conclude that the lateral line is uniquely suited among vertebrate sensory systems for a molecular, cellular and genetic analysis of pattern formation and of neural development.

0 Followers
 · 
80 Views
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Loss of the mechanosensory hair cells in the auditory and vestibular organs leads to hearing and balance deficits. To investigate initial, in vivo events in aminoglycoside-induced hair cell damage, we examined hair cells from the lateral line of the zebrafish, Danio rerio. The mechanosensory lateral line is located externally on the animal and therefore allows direct manipulation and observation of hair cells. Labeling with vital dyes revealed a rapid response of hair cells to the aminoglycoside neomycin. Similarly, ultrastructural analysis revealed structural alteration among hair cells within 15 minutes of neomycin exposure. Animals exposed to a low, 25-microM concentration of neomycin exhibited hair cells with swollen mitochondria, but little other damage. Animals treated with higher concentrations of neomycin (50-200 microM) had more severe and heterogeneous cellular changes, as well as fewer hair cells. Both necrotic-like and apoptotic-like cellular damage were observed. Quantitation of the types of alterations observed indicated that mitochondrial defects appear earlier and more predominantly than other structural alterations. In vivo monitoring demonstrated that mitochondrial potential decreased following neomycin treatment. These results indicate that perturbation of the mitochondrion is an early, central event in aminoglycoside-induced damage.
    The Journal of Comparative Neurology 06/2007; 502(4):522-43. DOI:10.1002/cne.21345 · 3.51 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The aim of this study is to shed light on the functional role of slc7a6os, a gene highly conserved in vertebrates. The Danio rerio slc7a6os gene encodes a protein of 326 amino acids with 46% identity to human SLC7A6OS and 14% to Saccharomyces cerevisiae polypeptide Iwr1. Yeast Iwr1 specifically binds RNA pol II, interacts with the basal transcription machinery and regulates the transcription of specific genes. In this study we investigated for the first time the biological role of SLC7A6OS in vertebrates. Zebrafish slc7a6os is a maternal gene that is expressed throughout development, with a prevalent localization in the developing central nervous system (CNS). The gene is also expressed, although at different levels, in various tissues of the adult fish. To determine the functional role of slc7a6os during zebrafish development, we knocked-down the gene by injecting a splice-blocking morpholino. At 24 hpf morphants show morphological defects in the CNS, particularly the interface between hindbrain and midbrain is not well-defined. At 28 hpf the morpholino injected embryos present an altered somite morphology and appear partially or completely immotile. At this stage the midbrain, hindbrain and cerebellum are compromised and not well defined compared with control embryos. The observed alterations persist at later developmental stages. Consistently, the expression pattern of two markers specifically expressed in the developing CNS, pax2a and neurod, is significantly altered in morphants. The co-injection of embryos with synthetic slc7a6os mRNA, rescues the morphant phenotype and restores the wild type expression pattern of pax2a and neurod. Our data suggest that slc7a6os might play a critical role in defined areas of the developing CNS in vertebrates, probably by regulating the expression of key genes.
    PLoS ONE 03/2015; 10(3):e0119696. DOI:10.1371/journal.pone.0119696 · 3.53 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: An important function of the vertebrate nervous system is chemosensation, and the ability to detect and distinguish between harmful and useful compounds and react appropriately. However, the neural mechanisms underlying chemosensory responses in vertebrates are poorly understood. Studies addressing chemosensory mechanisms would be greatly facilitated by using a vertebrate model that is easy to stimulate, has a simple well-characterized nervous system, and in which neural activity can be easily detected. Here, I present evidence for the first time demonstrating that the zebrafish lateral line can act as a chemosensory system. The lateral line is a hydrodynamic sensory system found in fish and amphibians. It is comprised of superficial sensory structures called neuromasts that are innervated by afferent and efferent nuclei in the brain. Using c-fos induction as a marker for neural activity I show that neuromasts, as well as neurons deep in the brain, respond to specific pungent natural compounds normally associated with nociception, or the sensation of pain. We take advantage of the optical transparency of the zebrafish larvae to functionally image the response using an optical imaging approach that we have developed. Using this approach we can detect neural activity in higher order components of the lateral line system in response to noxious stimuli. Our results assign an additional sensory role for the lateral line system in zebrafish and demonstrate the potential of this system for studying neural mechanisms associated with nociception in vertebrates, including humans. .

Preview

Download
1 Download
Available from