Article

Sauchinone, a lignan from Saururus chinensis, suppresses iNOS expression through the inhibition of transactivation activity of RelA of NF-kappaB.

Anticancer Agents Research Laboratory, Korea Research Institute of Bioscience and Biotechnology, Yuseong, Daejeon, Korea.
Planta Medica (Impact Factor: 2.35). 01/2004; 69(12):1096-101. DOI: 10.1055/s-2003-45189
Source: PubMed

ABSTRACT Sauchinone, a known lignan, was isolated from the root of Saururus chinensis as an active principle responsible for inhibiting the production of NO in LPS-stimulated RAW264.7 cells by activity-guided fractionation. Sauchinone dose-dependently inhibited not only the production of NO, but also the expression of iNOS mRNA and protein in LPS-stimulated RAW 264.7 cells. Furthermore, sauchinone prevented LPS-induced NF-kappaB activation, which is known to play a critical role in iNOS expression, assessed by a reporter assay under the control of NF-kappaB. However, an electrophoretic mobility shift assay (EMSA) demonstrated that sauchinone did not suppress the DNA-binding activity of NF-kappaB or the degradation of IkappaB-alpha induced by LPS. Further analysis revealed that transactivation activity of RelA subunit of NF-kappaB was dose-dependently suppressed in the presence of sauchinone. Taken together, our results suggested that sauchinone could inhibit production of NO in LPS-stimulated RAW264.7 cells through the suppression of NF-kappaB by inhibiting transactivation activity of RelA subunit.

0 Bookmarks
 · 
38 Views
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: This study was carried out to investigate the qualities of Saururus chinensis Baill. rhizomes and it content of active ingredient accenting to the storage methods. As the storage period increased the rates of weight loss, rotten rhizomes and the number of sprouts increased. Refrigerator storage resulted in the lowest weight reduction and sprouts rates, while storage in soil-filled box in a store-house was most effective in reducing spoilage rate. Five lignans from rhizome were determined by HPLC Retention time ranged minutes and showed saucernetin sauchinone, manassantin A, saucerneol D, and manassantin B in that order. Regardless of storage methods, the lignan content was lower after 120 days than after 30 days of storage, and increased manassantin B, manassantin A, saucernetin sauchinone, and saucerneol D in that order.
    Korean Journal of Food Preservation. 01/2006; 13(3).
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Nuclear factor kappa B (NF-κB) is a transcription factor involved at the downstream stage of many signalling cascades and plays a crucial role in acute and chronic inflammatory conditions, induced and adaptive immunity, apoptosis and induced cell proliferation. For the last decade it has attracted intense research interest throughout the world. This review briefly expands on information on biogenic modulators of this transcription factor published since an earlier review in 2002 and goes into much more detail on parthenolide and curcumin – two natural products that have been at the centre of molecular and biochemical interest in identifying modulators of the NF-κB pathway. Both compounds have attracted considerable attention in regard to the mechanistic aspects of the compounds’ action. An attempt to further develop some compounds into leads for new medicines are also discussed, focusing on an EU-funded project on anti-inflammatory natural products, mostly from medicinal plants used locally in selected Mediterranean regions.
    Phytochemistry Reviews 12/2004; 4(1):27-37. · 4.15 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Activity-guided fractionation of an 80% EtOH extract from the aerial parts of Saururus chinensis led to isolation of three anti-proliferative neolignans (1-3) along with four flavonoids (4-7) and four aristolactams (8-11). Their chemical structures were identified by analysis of spectroscopic data. All compounds 1-11 were evaluated for their activities against 28 human cancer cell lines using an in vitro cell proliferation assay. Compounds 1-3 showed potent anti-proliferative activities against cervical (C33a, IC50=0.01 µM for 1; 0.28 µM for 2; 2.80 µM for 3) and lung (NCI-H460, IC50=0.05 µM for 1; 1.37 µM for 2; 6.46 µM for 3) cancer cells without any remarkable cytotoxic effects on human normal lung cells as a control. Taken together, these data demonstrated the identification of anti-proliferative neolignans which are active components of S. chinensis.
    Biological & Pharmaceutical Bulletin 01/2012; 35(8):1361-6. · 1.85 Impact Factor