Article

Enrichment of hyperforin from St. John's wort (Hypericum perforatum) by pilot-scale supercritical carbon dioxide extraction.

Department of Pharmaceutical Technology, Eberhard-Karls-University Tübingen, Auf der Morgenstelle 8, D-72076 Tuebingen, Germany.
European Journal of Pharmaceutical Sciences (impact factor: 3.21). 04/2004; 21(4):443-51. DOI:10.1016/j.ejps.2003.10.026
Source: PubMed

ABSTRACT St. John's Wort (Hypericum perforatum L.) was extracted with supercritical carbon dioxide using a pilot batch extraction plant. The effects of pressure, temperature, flow rate and extraction time were examined with respect to extraction yield and hyperforin content. Supercritical carbon dioxide showed a high selectivity for phloroglucinols. Extracts were analyzed using an isocratic HPLC method with a mixture of hyperforin/adhyperforin as an external standard. Within the studied range of extraction pressure (90-150 bar) and extraction time (1-5 h), extraction at 90 bar for 3 h and 120 bar for 1 h provided higher hyperforin content (up to 35%) in the resulting extracts. An increase in extraction temperature showed a negative effect, leading to increased degradation of hyperforin into orthoforin. When the total mass of carbon dioxide passing the extraction vessel was kept constant, changes in mass flow rate did not affect the extraction result.

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  • Article: Isolation and purification of series bioactive components from Hypericum perforatum L. by counter-current chromatography.
    Xueli Cao, Qiaoe Wang, Yan Li, Ge Bai, Hong Ren, Chunming Xu, Yoichiro Ito
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Keywords

carbon dioxide
 
degradation
 
external standard
 
extraction
 
extraction pressure
 
extraction result
 
extraction temperature
 
extraction time
 
extraction vessel
 
extraction yield
 
higher hyperforin content
 
hyperforin content
 
Hypericum perforatum L
 
isocratic HPLC method
 
John's Wort
 
mass flow rate
 
pilot batch extraction plant
 
studied range
 
supercritical carbon dioxide
 
total mass
 

Harald Römpp