Anti-diarrheal and spasmolytic activities and acute toxicity study of Soonkijangquebo, a herbal anti-diarrheal formula
ABSTRACT The anti-diarrheal and spasmolytic activities of Soonkijangquebo (SKJQB), a Korean herbal anti-diarrheal formulation, were subjected to pharmacological evaluation. SKJQB, at a dose of 50-200 mg/kg, inhibited castor oil-induced diarrhea in mice. The median effective dose (ED50) for the anti-diarrheal effect was 93 mg/kg. In isolated rabbit jejunum preparations, SKJQB produced a spasmolytic effect by the relaxation of spontaneous contractions in a dose-dependent manner. The median effective concentration (EC50) for the spasmolytic effect was 3.6 mg/ml. In isolated guinea pig ileum preparations, SKJQB also produced a spasmolytic effect by reduction of acetylcholine-induced contractions. When tested against calcium channel blockade in rabbit jejunum, SKJQB caused a dose-dependent rightward shift in the Ca2+ dose-response curves, similar to that produced by verapamil, a well-known calcium antagonist. In an acute toxicity study in Sprague-Dawley rats, the median lethal dose (LD50) of SKJQB was greater than 2000 mg/kg, and no pathological changes were noticed in macroscopic examination by necropsy of rats treated with SKJQB. Thus, SKJQB may be safely used as a spasmolytic as well as an anti-diarrheal agent.
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ABSTRACT: Diarrhoea is a common public health problem, especially in developing countries, where the antidiarrhoeal medicines are not available for the whole population and could cause undesirable secondary effects. In many countries, the use of medicinal plants is a common alternative treatment for diarrhoeal diseases. In order to know the condition of the research on plant kinds which present antispasmodic and/or antidiarrhoeal properties, 242 selected studies developed between 192 and 2008 were reviewed. The 319 analyzed plant kinds were included in 101 botanical fami-lies, mostly Asteraceae, Fabaceae y Labiatae. Experiments were carried out in vitro more often than in vivo, using preferably isolated guinea-pig ileum, isolated rabbit jejunum, charcoal meal as intestinal motility marker and castor oil induced-dia-rrhoea. Antispasmodic compounds were mainly flavonoids, terpenes and alkaloids. Nine studies on antidiarrhoeal herbal medicine were observed. Most of the research was carried out in Asia; most of the total-extract studies were observed in Asia and Africa; and most of the isolated compound studies were registered in Europe. In conclusion, the research on medicinal plants with antispasmodic and/or antidia-rrhoeal agents contributes to solve diarrhoeal illness problem.
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ABSTRACT: We evaluated the pharmacological properties and spasmogenic activities of Yumijangquebotrade mark, a Korean herbal laxative formulation. Doses in the range 12-50 microg/ml induced a large spasmogenic effect in isolated guinea pig ileum, similar to that induced by acetylcholine. Pre-treating the tissue with atropine (0.2 microM) completely abolished the contractile effect of Yumijangquebo. The spasmogenic effect of Yumijangquebo and the inhibition of this effect by atropine suggest that a cholinergic mechanism is responsible for its effects. Yumijangquebo increased the gastrointestinal motility in ICR mice at doses between 10 and 37 mg/kg. Yumijangquebo exhibited higher activity than three other laxatives tested, which had activities about 85% of that of Yumijangquebo. In an acute toxicity study using Sprague-Dawley rats, the median lethal dose (LD50) of Yumijangquebo was greater than 2000 mg/kg, and we found no pathological changes in macroscopic examination by necropsy of rats treated with Yumijangquebo. We conclude that Yumijangquebo may be safely used as a herbal spasmogenic laxative agent.Journal of Ethnopharmacology 11/2005; 101(1-3):197-203. DOI:10.1016/j.jep.2005.04.016