Article

Seeing 'cool' and 'hot'--infrared thermography as a tool for non-invasive, high-throughput screening of Arabidopsis guard cell signalling mutants.

Department of Biological Sciences, Institute of Environmental and Natural Sciences, Lancaster University, Bailrigg, Lancaster LA1 4YQ, UK.
Journal of Experimental Botany (impact factor: 5.36). 06/2004; 55(400):1187-93. DOI:10.1093/jxb/erh135 pp.1187-93
Source: PubMed

ABSTRACT The use of Arabidopsis mutants defective in abscisic acid (ABA) perception has been instrumental in the understanding of stomatal function, in particular, ABA signalling in guard cells. The considerable attention devoted to ABA signalling in guard cells is due in part to (1) the fundamental role of ABA in drought stress and (2) the use of a screening protocol based on the sensitivity of seed germination to ABA. Such a screen has facilitated the isolation of ABA signalling mutants with genetic lesions that exert pleiotropic effects at the whole plant level. As such, there is a requirement for new approaches to complement the seed germination screen. The recent advances made in the use of infrared thermography as a non-invasive, high-throughput tool are reviewed here and the versatility of this technique for screening Arabidopsis defective in stomatal regulation is highlighted.

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    PLoS ONE 01/2011; 6(5):e20243. · 4.09 Impact Factor
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Keywords

ABA
 
ABA signalling
 
ABA signalling mutants
 
Arabidopsis mutants defective
 
exert pleiotropic effects
 
fundamental role
 
genetic lesions
 
guard cells
 
high-throughput tool
 
infrared thermography
 
new approaches
 
screening Arabidopsis defective
 
seed germination
 
seed germination screen
 
stomatal function
 
stomatal regulation
 
whole plant level