Comparison of leptin gene expression in different adipose tissues in children and adults

Department of Pediatrics, University of Erlangen-Nuremberg, Loschgestrasse 15, 91054 Erlangen, Germany.
European Journal of Endocrinology (Impact Factor: 4.07). 05/2004; 150(4):579-84. DOI: 10.1530/eje.0.1500579
Source: PubMed

ABSTRACT Adipose tissue displays depot-specific metabolic properties and a predominant gene expression of leptin in subcutaneous tissue. The aim of the study was to evaluate leptin mRNA expression in various adipose tissues and to relate it to plasma leptin concentrations. Furthermore, developmental changes in leptin gene expression from childhood to adulthood were examined.
Thoracic subcutaneous and intrathoracic adipose tissue specimens were obtained in 22 adults (51-81 years) and 23 children (0.1-17 years) undergoing cardiac surgery, and abdominal subcutaneous, omental and mesenterial fat specimens were collected from 21 adults (38-79 years) and 22 children (0.2-17 years) before abdominal surgery. Preoperative plasma leptin concentrations were measured by RIA. Leptin mRNA expression was quantified by TaqMan real-time PCR.
In adults, there was no difference between leptin gene expression in subcutaneous and intrathoracic fat, whereas in children leptin mRNA expression was significantly higher in subcutaneous adipose tissue. In omental fat, leptin mRNA levels were significantly lower compared with subcutaneous and mesenterial sites in both children and adults. Adults revealed a significantly higher leptin gene expression in subcutaneous, omental and mesenterial adipose tissues than children. Subcutaneous and omental leptin gene expression are independent factors for plasma leptin concentrations in children and adults.
Leptin is differentially expressed at different adipose tissue sites, a situation which is even more pronounced in children. There is a developmental increase in leptin mRNA expression in adipose tissue during childhood, reaching maximal capacity in adulthood.

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    • "These observations are in agreement with few reports which showed strong correlation between the levels of leptin mRNA expression and adipocyte size supporting the importance of leptin in altering the fat mass [19,20]. Another study on human subjects showed that the developmental increase in leptin mRNA expression in adipose tissue during childhood, reaching maximal capacity in adulthood [21]. In addition a study also depicted an evidence linking leptin to a direct regulation of adipose tissue metabolism through inhibition of lipogenesis and stimulation of lipolysis [22]. "
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    Lipids in Health and Disease 02/2014; 13(1):25. DOI:10.1186/1476-511X-13-25 · 2.22 Impact Factor
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    • "Regardless, leptin expression traits distinguish adipose depots in several species including man (Table 4, Li et al., 2007; Schoof et al., 2004). Leptin gene expression is greater in the subcutaneous depot of several species (Table 4) as it is in the subcutaneous depot of children (1–17 years old) and adults (Li et al., 2007; Schoof et al., 2004). "
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    Molecular and Cellular Endocrinology 12/2009; 323(1):20-34. DOI:10.1016/j.mce.2009.12.011 · 4.41 Impact Factor
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    • "Schoof et al. natomiast stwierdzili większą u osób doro− słych niż u dzieci ekspresję mRNA leptyny w tkance tłuszczowej podskórnej oraz tkance tłu− szczowej sieci i krezki. Stężenie mRNA leptyny w tkance tłuszczowej podskórnej wykazywało do− datnią korelację ze stężeniem leptyny we krwi i to niezależnie od wieku badanych [24]. Fajardo et al. wykazali, że w porównywalnej w odniesieniu do BMI grupie kobiet stężenie mRNA leptyny w podskórnej tkance tłuszczowej jest istotne większe w okresie przed menopauzą aniże− li po okresie menopauzy [25]. "
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