Multipotency of Flk1+CD34- progenitors derived from human fetal bone marrow

State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Nanjing Road 288, Tianjin 300020, People's Republic of China.
Journal of Laboratory and Clinical Medicine (Impact Factor: 2.8). 05/2004; 143(4):230-40. DOI: 10.1016/j.lab.2003.11.008
Source: PubMed


We report that a cell population derived from human fetal bone marrow, termed Flk1+CD34- multipotent stem cells, can differentiate not only into osteogenic, adipogenic, and endothelial lineages but also into hepatocyte-like cells and neural and erythroid cells at the single-cell level. We depleted mononuclear cells from fetal bone marrow of CD45+, GlyA+, and CD34+ cells with the use of micromagnetic beads, then cultured them by limiting dilution. Three single colonies were harvested, expanded, and characterized. The clones have been expanded for more than 50 cell doublings, and cell-doubling time was about 30 hours. About 90% cells were in the G(0)/G(1) phase of the cell cycle, and the cells from the single colony maintained Flk1+ and CD34-. Because fetal bone marrow-derived Flk1+CD34-multipotent stem cells have the capacity for self-renewal and multilineage differentiation even after being expanded for more than 50 cell doublings, they may be an ideal source of stem cells for the treatment of inherited or degenerative diseases.

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    • "Therefore, it is of great importance for scientists to find adult stem cells that have a similar differentiation potential to embryonic stem cells. Researchers led by Zhao at the Chinese Academy of Medical Sciences reported that a cell population derived from human foetal bone marrow, termed Flk1 C CD31 K CD34 K stem cells, could differentiate, not only into osteogenic, adipogenic and endothelial lineages, but also hepatocyte-like, neural and erythroid cells at the single-cell level (Fang et al. 2003, 2004). Zhao et al. used cells from a single colony, which precluded the possibility of contamination by different stem cells. "
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    • "Results showed that adherent Flk1 + CD31 À CD34 À cells can also be isolated from adipose tissue and be continuously passaged for at least 20 passages. The morphology , phenotype, and in vitro differentiation potential of the ADAS cells are similar to those of fetal BM-derived Flk1 + CD31 À CD34 À MSCs [7] [23]. They could differentiate into osteogenic and adipogenic cells in vitro. "
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