Detection of preceding Campylobacter jejuni infection by polymerase chain reaction in patients with Guillain-Barré syndrome.

Department of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow 226 014, India.
Transactions of the Royal Society of Tropical Medicine and Hygiene (Impact Factor: 1.82). 06/2004; 98(6):342-6. DOI: 10.1016/j.trstmh.2003.10.007
Source: PubMed

ABSTRACT Based on culture and serological evidence, a strong association between Campylobacter jejuni infection and Gullain-Barré syndrome (GBS) has been established. However, culture underestimates C. jejuni infection in GBS and the specificity of serology remains uncertain. Thus, a direct sensitive detection method for recent C. jejuni infection is required. We used the PCR technique in GBS patients to assess its role in the diagnosis of C. jejuni infection. From June 2001 to March 2003, stool specimens from 42 patients with GBS and an equal number of age- and gender-matched healthy controls were analysed for C. jejuni infection by culture and PCR. Gullain-Barré syndrome subtypes were classified by clinical and electrophysiological studies. Of the GBS patients, two (4.8%) and eight (19%) were positive by culture and PCR, respectively, and the difference was significant (P < 0.05). None of the controls were positive for C. jejuni by culture or PCR. All C. jejuni-positive GBS patients had axonal degeneration with or without sensory involvement. The incidence of C. jejuni-associated GBS cases was more frequent during summer than winter (7/19, 36.8% vs. 1/23, 4.3%, P < 0.01). Polymerase chain reaction appears to be a sensitive tool to detect preceding C. jejuni infection in GBS patients.

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    ABSTRACT: :Campylobacteriosis -caused principally by Campylobacter jejuni (C. jejuni) and Campylobacter coli (C. coli) - is among the main causes of bacterial gastroenteritis worldwide. This work was done to investigate the molecular characterization of zoonotic C. jejuni and C. coli isolated from fecal samples of beef cattle, retail beef meat and beef liver and stool of children with diarrhea. Fecal samples were collected from 50 apparently healthy cattle, 60 of retail beef meat and beef liver (30 of each) as well as 50 stool samples from pediatric diarrhea were subjected to standard isolation and phenotypic identification of Campylobacter isolates. The prevalence of Campylobacter isolate was 17(34%) in fecal sample of cattle, 5(16.66%) beef meat, 8(26.66 %) beef liver and 13 (26%) in pediatric diarrhea. Out of 43 identified isolates, 26(60.46%) C. jejuni isolates were higher than 14(32.55%) C. coli, two samples were mixed infection and one Campylobacter upsaliensis. A multiplex-PCR method was developed for the detection of C. jejuni and C. coli. Primers were the hippuricase gene (hipO) characteristic of C. jejuni, a sequence partly covering an aspartokinase gene (asp) characteristic of C. coli and a universal 16S rDNA gene sequence serving as an internal positive control. All Campylobacter isolates expressed identity with 16S rDNA (genus specific gene) at 1062 pb. Multiplex PCR demonstrated one false- positive and one false-negative hippurate activity test. PCR method was incapable to identify biochemically identified C. upsaliensis. Amplification of hipO gene of C. jejuni and asp- gene of C. coli isolated from cattle, beef and liver have shown identical fingerprints with human C. jejuni and C. coli at 344bp and 500bp respectively, indicating the public health importance of the isolates