A CHASE domain containing protein kinase OsCRL 4, represents a new AtCRE 1-like gene family in rice

Stake Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China.
Journal of Zhejiang University SCIENCE 07/2004; 5(6):629-33. DOI: 10.1007/BF02840972
Source: PubMed


AtCRE1 is known to be a cytokinin receptor in Arabidopsis. The AtCRE1 protein contains CHASE domain at the N-terminal part, followed by a transmitter (histidine kinase) domain and two receiver domains. The N-terminal CHASE domain of AtCRE1 contains putative recognition sites for cytokinin. Five CHASE domains containing proteins were found in rice, OsCRL1a, OsCRL1b, OsCRL2, OsCRL3, and OsCRL4. OsCRL1a, OsCRL1b, OsCRL2 and OsCRL3 contain the four domains existing in CRE1, whereas OsCRL4 only contains the CHASE domain and a putative Ser/Thr protein kinase domain. The authors cloned the encoding gene OsCRL4 and found that it represents a new member of the cytokinin receptor protein in rice.

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    • "An alternative, but not mutually exclusive, possibility is that there are additional cytokinin receptors present in plants. Several other potential cytokinin receptors have been suggested, including a seven-pass transmembrane protein (Plakidou-Dymock et al., 1998) and several cytokinin-binding proteins (Kulaeva et al., 1998), and in rice (Oryza sativa), there is a gene encoding a protein with a predicted CHASE domain fused to a Ser/Thr kinase intracellular domain (Han et al., 2004). However, it remains to be seen if these other proteins serve as authentic cytokinin receptors. "
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    • "The four OsHK proteins possess three conserved domains (CHASE domain, transmitter domain, and receiver domain) and two or more transmembrane domains (Fig. 1). However, OsHKL1 (OsCRL4) [22] possesses only the CHASE motif. All OsHKs have the conserved Thr that characterizes the CHASE motif and mutation of Thr to Ile in Arabidopsis AHK4 will lead to a loss of function [15]. "
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    ABSTRACT: In this report we define the genes of two-component regulatory systems in rice through a comprehensive computational analysis of rice (Oryza sativa L.) genome sequence databases. Thirty-seven genes were identified, including 5 HKs (cytokinin-response histidine protein kinase) (OsHK1-4, OsHKL1), 5 HPs (histidine phosphotransfer proteins) (OsHP1-5), 15 type-A RRs (response regulators) (OsRR1-15), 7 type B RR genes (OsRR16-22), and 5 predicted pseudo-response regulators (OsPRR1-5). Protein motif organization, gene structure, phylogenetic analysis, chromosomal location, and comparative analysis between rice, maize, and Arabidopsis are described. Full-length cDNA clones of each gene were isolated from rice. Heterologous expression of each of the OsHKs in yeast mutants conferred histidine kinase function in a cytokinin-dependent manner. Nonconserved regions of individual cDNAs were used as probes in expression profiling experiments. This work provides a foundation for future functional dissection of the rice cytokinin two-component signaling pathway.
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