Article

Sequence-based typing of exons 1-5 of a new HLA-B allele, B*3927*.

Tissue Typing Laboratory, University Hospital Maastricht, 6202 AZ Maastricht, The Netherlands.
Tissue Antigens (impact factor: 2.59). 06/2004; 63(5):480-3. DOI:10.1111/j.0001-2815.2004.00178.x pp.480-3
Source: PubMed

ABSTRACT Anew human leucocyte antigen-B (HLA-B) allele, B*3927, was detected in three individuals of a Caucasian family by routine typing with sequence-specific primers (SSP). Serological typing showed B27 Bw4 and B39 Bw6, whereas SSP detected only B*27 as well as the Bw4 and Bw6 motif. The sequence of exons 1-5 of the new allele was determined by allele-specific amplification and sequencing. The new B*39 allele showed one nucleotide difference with B*390101 at position 299 in exon 2. Codon 100 changed from GAG to GTG, resulting in an amino acid substitution from glutamic acid to valine at position 76 of the mature protein. The haplotype carrying the B*3927 allele was A*010101, B*3927, Cw*120301, DRB1*0101 and DQB1*050101.

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Keywords

allele-specific amplification
 
amino acid substitution
 
Anew human leucocyte antigen-B
 
Bw6 motif
 
exons 1-5
 
GAG
 
glutamic acid
 
HLA-B
 
new allele
 
new B*39 allele
 
nucleotide difference
 
routine typing
 
sequence-specific primers
 

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