Histamine dilutions modulate basophil activation.

Boiron, 20 rue de la Libération, 69110 Sainte-Foy-Les-Lyon, France.
Inflammation Research (Impact Factor: 2.14). 06/2004; 53(5):181-8. DOI: 10.1007/s00011-003-1242-0
Source: PubMed

ABSTRACT In order to demonstrate that high dilutions of histamine are able to inhibit basophil activation in a reproducible fashion, several techniques were used in different research laboratories.
The aim of the study was to investigate the action of histamine dilutions on basophil activation.
Basophil activation was assessed by alcian blue staining, measurement of histamine release and CD63 expression. Study 1 used a blinded multi-centre approach in 4 centres. Study 2, related to the confirmation of the multi-centre study by flow cytometry, was performed independently in 3 laboratories. Study 3 examined the histamine release (one laboratory) and the activity of H(2) receptor antagonists and structural analogues (two laboratories).
High dilutions of histamine (10(-30)-10(-38) M) influence the activation of human basophils measured by alcian blue staining. The degree of inhibition depends on the initial level of anti-IgE induced stimulation, with the greatest inhibitory effects seen at lower levels of stimulation. This multicentre study was confirmed in the three laboratories by using flow cytometry and in one laboratory by histamine release. Inhibition of CD63 expression by histamine high dilutions was reversed by cimetidine (effect observed in two laboratories) and not by ranitidine (one laboratory). Histidine tested in parallel with histamine showed no activity on this model.
In 3 different types of experiment, it has been shown that high dilutions of histamine may indeed exert an effect on basophil activity. This activity observed by staining basophils with alcian blue was confirmed by flow cytometry. Inhibition by histamine was reversed by anti-H2 and was not observed with histidine these results being in favour of the specificity of this effect We are however unable to explain our findings and are reporting them to encourage others to investigate this phenomenon.

1 Bookmark
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Background Reproducibility of basic research investigations in homeopathy is challenging. This study investigated if formerly observed effects of homeopathically potentised gibberellic acid (GA3) on growth of duckweed (Lemna gibba L.) were reproducible. Methods Duckweed was grown in potencies (14x–30x) of GA3 and one time succussed and unsuccussed water controls. Outcome parameter area-related growth rate was determined by a computerised image analysis system. Three series including five independent blinded and randomised potency experiments (PE) each were carried out. System stability was controlled by three series of five systematic negative control (SNC) experiments. Gibbosity (a specific growth state of L. gibba) was investigated as possibly essential factor for reactivity of L. gibba towards potentised GA3 in one series of potency and SNC experiments, respectively. Results Only in the third series with gibbous L. gibba L. we observed a significant effect (p = 0.009, F-test) of the homeopathic treatment. However, growth rate increased in contrast to the former study, and most biologically active potency levels differed. Variability in PE was lower than in SNC experiments. The stability of the experimental system was verified by the SNC experiments. Conclusions Gibbosity seems to be a necessary condition for reactivity of L. gibba to potentised GA3. Further still unknown conditions seem to govern effect direction and the pattern of active and inactive potency levels. When designing new reproducibility studies, the physiological state of the test organism must be considered. Variability might be an interesting parameter to investigate effects of homeopathic remedies in basic research.
    Homeopathy: the journal of the Faculty of Homeopathy 04/2014; 103(2):113–126. · 1.13 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The most common way to perform succussions is to place a liquid preparation inside a glass vessel and beat it vigorously against a hard elastic surface, either manually or using a mechanical apparatus. This procedure has been assumed able to transfer mechanical energy to the molecular level, where it becomes available to perform chemical work. Such interpretation has been enforced by observed changes in the electrical conductivity (EC) of High Dilutions (HD) due to succussion. In order to address this question, we compared the electrical conductivity changes of HD prepared from Vincristine sulfate (VCR) samples with those of an inert solvent. Samples were produced through manual and mechanical succusions in order to observe the influence of bubbles production. The results confirmed the timing of EC changes but these were equivalent for VCR and solvent, except for VCR 1cH samples. Also, the production of bubbles does not affect the EC in an extent able to distinguish succussion procedures. We concluded that the physical-chemical properties of HD can be modeled by chemical and diffusive mechanisms typical of distilled water.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Background: In the preparation of ultra high dilutions (UHD) each dilution step is followed by a succussion. Whereas the physical basis of succussion have been well studied [1], there are only few papers regarding the effects of succussion number (NS) [2]. In previous studies [3, 4] we showed that arsenic at UHD applied on arsenic stressed wheat seeds stimulates the germination rate. Aims: Investigate whether NS applied between the dilution-steps influences the UHD effectiveness. Methodology: Plant material and stress treatment: Wheat seeds (Triticum aestivum L.) of cv. Pandas were stressed by 30min of poisoning with 5mM As2O3 aqueous solution [3, 4]. Classes of treatment: Undiluted and unsuccussed H2O (C), H2O and As2O3 both at the 45th decimal dilution/succussion (W45x and As45x, respectively). The succussion was handmade and NS differed for each treatment (NS= 4, 8, 16, 32, 40, 70, 100). Biological model: In each Petri dish 36 seeds were placed and watered with 20ml of treatment. After 96 hours non-germinated seeds were counted. The experiment was repeated twice and each one consisted in 6 Petri dishes per treatment and 6 trials. The results were analyzed by Poisson test [3]. Droplet evaporation method: The droplet patterns [5] were prepared out of the stressed wheat samples treated either with As45x, W45x (NS=8, 32, 70) or C. The experiment was performed in a 3 day repetition, 3 replicates per day, 5 droplets per replicate. The patterns were evaluated by means of the ImageJ software for their local connected fractal dimension (LCFD) values. The data was analyzed by means of the analysis of variance. Multiple mean comparison was carried out by Turkey’s HSD test. Results: NS strongly influenced both the biological effectiveness of the treatment (i) and the LCFD of the DEM patterns (ii). In particular: (i) the rate of germinated seeds significantly increased vs. C following treatments with NS≥32 for both As45x and W45x; (ii) all As45x (NS=8, 32, 70) and W45x with NS=70 significantly increased the LCFD of DEM patterns vs. C. Both approaches showed that UHD effects increase with the growing NS, even if there was a non complete correspondence of the significance levels. Conclusions: Our results show that NS is an important parameter influencing the UHD effectiveness.
    International Journal of High Performance Computing and Networking 09/2013; 12(44).

Full-text (2 Sources)

Available from
Jun 4, 2014