LC/MS/NMR analysis of isomeric divanilloylquinic acids from the root bark of Fagara zanthoxyloides Lam.
ABSTRACT Gradient HPLC coupled to DAD/UV, MS/MS and NMR has been applied to the rapid structure determination of three new isomeric divanilloylquinic acids from Fagara zanthoxyloides collected in Burkina Faso: 3,4-O-divanilloylquinic acid, 3,5-O-divanilloylquinic acid and 4,5-O-divanilloylquinic acid. Furthermore these new compounds named burkinabins A-C could play a useful role in sickle cell disease, as the active agents of Fagara zanthoxyloïdes are said to be unidentified aromatic compounds with carboxylic acid grouping (Adesanya, S.A., Sofowora, A., 1983. Biological standardisation of Zanthoxylum roots for antisickling activity. Planta Med. 48, 27-33).
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ABSTRACT: Sickle cell disease (SCD) is known to be one of the diseases wrecking most parts of the globe without any discrimination of ethnic or racial standards. It is characterized by a variety of symptoms including, shortness of breath, heart palpitations, abdominal pains, aches and pains in the muscle. The crisis stage is characterized by severe pain in the head and whole body. In the stage of crises if the percentage of sickled erythrocytes can be some how lowered we can expect a great relief to the patient. This could also serve a major step to-words management of the SCD. Phytochemicals in the plant extracts have the therapeutic activity and is used in traditional practice by the traditional healers. Many plant extracts have been used to bring about reversal of the sickled erythrocytes in vitro. The present communication reviews the available literature reporting anti-sickling properties of a number of plants extracts on human blood samples.world journal of phytology. 04/2012; 4:24-29.
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ABSTRACT: Sickle cell disease or drepanocytosis is caused by the polymerisation of abnormal haemoglobin S when oxygen tension decreases. This lead to the changes in the shape of red blood cells and anaemia. It has also been postulated that the red cells of patients with sickle cell disease contain a higher than normal concentration of calcium ions. These ions are bound to membrane proteins resulting in dehydration and loss of red blood cell deformability and cell-to-cell adherence. Anthocyanins extracted from some Congolese plants used in traditional medicine against sickle cell disease have recently been shown to have anti-sickling activity in vitro. Justicia secunda is a plant used in Congo by Jehovah's Witnesses, well known for their refusal of blood transfusions, against anaemia. Emmel, Itano and osmotic fragility tests were used to test the effect of anthocyanin extracts from Justicia secunda leaves on haemoglobin S solubility and sickle cell membrane stability. Anthocyanins from Justicia secunda were found to possess anti-sickling activity. Treated SS red blood cells recovered a normal, classical biconcave form with a radius of 3.3±0.3 μm, similar to that of normal erythrocytes. The solubility of deoxyhaemoglobin S increased and the osmotic fragility of drepanocytes decreased upon treatment with anthocyanin extracts. These findings suggest that anthocyanin extracts play a role in both stabilising the red blood cell membrane and inhibiting polymerisation of haemoglobin S. This provides a possible molecular basis for earlier reports on the anti-sickling properties of anthocyanins from some Congolese plants and their use in the management of sickle cell disease by Congolese traditional healers.Blood transfusion = Trasfusione del sangue 10/2010; 8(4):248-54. · 1.86 Impact Factor
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ABSTRACT: Aims: To evaluate the antisickling and radical scavenging activities and acute toxicity of indigenous nutritive formula Drepanoalpha®, produced through a bio-guided based plant selection. Study Design: Drepanoalpha® extracts, Antisickling activity by Emmel test, Antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl bleaching methods; acute toxicity on rats, determination of biological and haematological parameters. Place and Duration of Study: Science Faculty University of Kinshasa, between January 2013 and February 2014. Methodology: The antisickling and antioxidant activities of Drepanoalpha® were determined using Emmel and the 1,1-diphenyl-2-picrylhydrazyl bleaching methods respectively. Acute oral toxicity test was performed to determine the LD50. Liver and kidney functions, the hematological and histopathological examinations were assessed using standard techniques. Results: Obtained results revealed that Drepanoalpha® possessesinteresting in vitro antisickling and antioxidant activities as revealed by the observed normal biconcave form of sickle erythrocyte (normalization rate >80%) and the radical scavenging activity (ED50= 0.604 ± 0.028 μg/mL). Acute toxicity assessment revealed that the medium lethal dose (LD50) is higher than 4000 mg/kg. Drepanoalpha® significantly increases the values of WBC, RBC, Hb, HCT, PLT, IDR-CV and PCT. Furthermore, this polyherbal formula significantly decreases the values of IDR-SD, P-RGC, AST and ALT (p<0.05). Both the control and treated groups displayed comparable non altered histological architecture of the liver cells. Discussion: The mean values of biochemical markers and hematological markers of treated rats revealed that Drépanoalpha® is potentially safe indicating non-toxic effect of the phytomedicine on immune cells and blood clotting factors. Moreover, this poly-herbal formulation increases the hemoglobin rate in the all treated rats (500-4000 mg/kg bodyweight) and preserves the histological architecture of the liver cells. Conclusion: Drepanoalpha® may increase weight gain, promote erythropoiesis and thrombopoeisis in sicklers patients. This phytomedicine could be used in the treatment of all form of anemia and may also prevent bile duct obstruction or intra-hepatic cholestasis. The results can form the basis for clinical trials in humans.European Journal of Medicinal Plants. 07/2014; 4(10):1251-1267.