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Determination of bisphenol A in river water and body fluid samples by stir bar sorptive extraction with in situ derivatization and thermal desorption-gas chromatography-mass spectrometry

Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.
Journal of Chromatography B (Impact Factor: 2.69). 07/2004; 805(1):41-8. DOI: 10.1016/j.jchromb.2004.02.005
Source: PubMed

ABSTRACT A new method, based on stir bar sorptive extraction (SBSE) with in situ derivatization and thermal desorption (TD)-gas chromatography-mass spectrometry (GC-MS) is described for the determination of trace amounts of bisphenol A (BPA) in river water, urine, plasma, and saliva samples. The derivatization conditions with acetic acid anhydride and the SBSE conditions such as sample volumes and extraction time are investigated. Then, the stir bar is subjected to TD followed by GC-MS. The detection limits of BPA in river water, urine, plasma, and saliva samples are 1-5, 20, 100, and 20pgml(-1) (ppt), respectively. Calibration for BPA was shown to be linear with a correlation coefficient of >0.99. The average recoveries of BPA in all samples are higher than 95% (R.S.D. < 10%) with correction using an added surrogate standard, 13C12-bisphenol A. This simple, accurate, sensitive, and selective analytical method may be applicable to the determination of trace amounts of BPA in liquid samples.

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    • "In agreement with previous data [21] [23], the sensitivity for BPs increased between 5 and 10-fold when using EG-Silicone stir bars compared with the obtained with those coated with PDMS and preconcentrating the analytes not derivatized. Nevertheless, the preconcentration of underivatized BPs using EG-Silicone SBSE stir bars is still less sensitive than those methods that include derivatization , such as in-tube silylation or in situ acetylation [19–23,29], although the method described here can be considered simpler than those involving derivatization since derivatization reagents are not used. "
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    Journal of pharmaceutical and biomedical analysis 02/2013; 78-79C:255-260. DOI:10.1016/j.jpba.2013.02.023 · 2.83 Impact Factor
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    • "Biomatrix Drug / metabolite / biomarker Extraction phase ( thickness ( mm ) , volume ( mL ) Commercial availability Method type Sample volume Analytical technique Desorption type Sensitivity ( LOQ , ng / mL ) Reference Urine Bisphenol A PDMS ( 0 . 5 , 24 ) Yes HS - SBSE 2 mL GC - MS TD 0 . 1 Kawaguchi et al . , 2004a"
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    • "The methods most frequently used for the determination of bisphenol A in environmental water are gas chromatography (GC) (Kawaguchi et al., 2004; Liu et al., 2004) and liquid chromatography (LC) (Shin et al., 2001; Watabe et al., 2004), both coupled to mass spectrometry (MS). These methods are highly sensitive and specific, but require time-consuming pre-treatment steps and thus do not allow rapid processing of multiple samples. "
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