Determination of bisphenol A in river water and body fluid samples by stir bar sorptive extraction with in situ derivatization and thermal desorption-gas chromatography-mass spectrometry.
ABSTRACT A new method, based on stir bar sorptive extraction (SBSE) with in situ derivatization and thermal desorption (TD)-gas chromatography-mass spectrometry (GC-MS) is described for the determination of trace amounts of bisphenol A (BPA) in river water, urine, plasma, and saliva samples. The derivatization conditions with acetic acid anhydride and the SBSE conditions such as sample volumes and extraction time are investigated. Then, the stir bar is subjected to TD followed by GC-MS. The detection limits of BPA in river water, urine, plasma, and saliva samples are 1-5, 20, 100, and 20pgml(-1) (ppt), respectively. Calibration for BPA was shown to be linear with a correlation coefficient of >0.99. The average recoveries of BPA in all samples are higher than 95% (R.S.D. < 10%) with correction using an added surrogate standard, 13C12-bisphenol A. This simple, accurate, sensitive, and selective analytical method may be applicable to the determination of trace amounts of BPA in liquid samples.
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ABSTRACT: Bisphenol A (BPA) is a widely used industrial chemical in the manufacturing of polycarbonate plastic bottles, food and beverage can linings, thermal receipts, and dental sealants. Animal and human studies suggest that BPA may disrupt normal hormonal function and hence, potentially, have negative effects on the human health. While total BPA is frequently reported, it is recognized that free BPA is the biologically active form and is rarely reported in the literature. The objective of this study was to develop a sensitive and improved method for the measurement of free and total BPA in human urine. Use of a labeled conjugated BPA (bisphenol A-d6 β-D-glucuronide) allowed for the optimization of the enzymatic reaction and permitted an accurate determination of the conjugated BPA concentration in urine samples. In addition, a (13)C12-BPA internal standard was used to account for the analytical recoveries and performance of the isotope dilution method. Solid-phase extraction (SPE) combined with derivatization and analysis using a triple quadrupole GC-EI/MS/MS system achieved very low method detection limit of 0.027 ng/mL. BPA concentrations were measured in urine samples collected during the second and third trimesters of pregnancy in 36 Canadian women. Total maternal BPA concentrations in urine samples ranged from not detected to 9.40 ng/mL (median, 1.21 ng/mL), and free BPA concentrations ranged from not detected to 0.950 ng/mL (median, 0.185 ng/mL). Eighty-six percent of the women had detectable levels of conjugated BPA, whereas only 22 % had detectable levels of free BPA in their urine. BPA levels measured in this study agreed well with data reported internationally.Analytical and Bioanalytical Chemistry 05/2014; · 3.66 Impact Factor
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ABSTRACT: A simple and highly sensitive electroanalytical method for the determination of bisphenol F (BPF) was developed, which was carried out on multi-walled carbon nanotubes-COOH (MWCNT-COOH) modified glassy carbon electrode (GCE) using cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The results showed that MWCNT-COOH remarkably enhanced the oxidation of BPF, which improved the anodic peak current of BPF significantly. The mechanism was oxidation of BPF lose electrons on the electrode surface via adsorption-controlled process, electrode reaction is the two electrons/two protons process. Under the optimised conditions, the oxidation peak current was proportional to BPF concentration the range from 0.12 to 6.01μgmL(-1). The detection limit was 0.11μgmL(-1) (S/N=3), and the relative standard deviation (R.S.D.) was 3.5% (n=9). Moreover, the MWCNT-COOH/GCE electrode showed good reproducibility, stability and anti-interference. Therefore, the proposed method was successfully applied to determine BPF in food packing and the results were satisfactory.Food Chemistry 08/2014; 157C:464-469. · 3.26 Impact Factor
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ABSTRACT: Bisphenol A found in plastic vessels used for children feeding is an endocrine disrupting compound. Therefore it can also induce the obesity at a very early stage in the life of children, and its presence in children saliva should be checked. We proposed eight microsensors: four stochastic microsensors, one amperometric microsensor and three multimode microsensors for the screening of children saliva for bisphenol A in a concentration range from 10−15 to 10−4 mol/L. Qualitative assessment of bisphenol A in saliva samples was done using stochastic sensing, while its quantitative assessment was done using differential pulse voltammetry and stochastic sensing. The results correlated well, and also compared with those obtained using the standard method, although the standard method did not cover all the time the ranges on which bisphenol A is present in children saliva, and therefore the standard method cannot be reliable used for it analysis in children saliva.Analytical Chemistry Research. 08/2014; 1:1–7.