HIV-1 CRF01_AE in intravenous drug users in Hanoi, Vietnam

National Institute of Hygiene and Epidemiology, Hanoi, Vietnam.
AIDS Research and Human Retroviruses (Impact Factor: 2.33). 04/2004; 20(3):341-5. DOI: 10.1089/088922204322996581
Source: PubMed


To investigate the molecular epidemiology of HIV-1 among intravenous drug users (IDUs) in Hanoi we collected 17 samples from individuals living in 12 locations in and around Hanoi. The HIV-1 env V3 and gag p17 regions were directly sequenced from the proviral PBMC population. The majority of the IDUs were infected with HIV-1 CRF01_AE and one individual carried a p17/V3 CRF01/subtype C recombinant. The CRF01 viruses found among these individuals did not seem to be directly epidemiologically linked to each other. The sequences were, however, related to previously reported CRF01 sequences from Vietnam and China. Thus, IDUs in Hanoi seem to have derived their infections in Vietnam, but not from the same source. The discovery of the CRF01/C recombinant shows that new viral forms easily can be generated in IDU transmission chains.

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    • "The final dataset was composed of 1,957 CRF01_AE p17 sequences sampled from blood and collected between 1990-2010, which represented 15 countries (Table 1). Sequences were obtained through the utilization of PBMCs, serum or plasma samples of HIV-1 positive individuals, and methods of extraction and amplification were deliberately detailed in their relative studies [6,8,16-20]. All sequences were aligned with HIV-1 subtype reference sequences ( "
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    ABSTRACT: HIV-1 CRF01_AE accounts for an important fraction of HIV infections in Asia including China, but little is known about the phylogenetic and evolutionary history of this CRF (circulating recombinant form). In the current study, we collected a large number of 1,957 CRF01_AE gag p17 sequences with known sampling year (1990-2010) from 5 global regions representing 15 countries to better understand the phylogenetic relationships and epidemic history of CRF01_AE strains in China. CRF01_AE gag p17 sequences were retrieved from public databases to explore phylogenetic relationships and phylogeographic dynamics of CRF01_AE in Asia by using maximum-likelihood phylogenetics and Bayesian coalescent-based analyses. We found close phylogenetic relationships between sequences from Thailand, Vietnam and China. Moreover, at least 5 independent introductions and 5 independent autochthonous clades of CRF01_AE, which descended from Thailand or Vietnam were identified in China from 1991 through 2003. The current study not only defines the migration of CRF01_AE clades to/in Asia, but also demonstrates the criticalness of identifying the circulating strains in the population for the development of vaccine and microbicides.
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