Fluconazole and itraconazole susceptibility of vaginal yeast isolates from Slovakia

1st Department of Obstetrics and Gynecology, Faculty of Medicine, Comenius University, Zochova 7, 811 03 Bratislava, Slovak Republic.
Mycopathologia (Impact Factor: 1.55). 03/2004; 157(2):163-9. DOI: 10.1023/B:MYCO.0000020594.35357.b0
Source: PubMed

ABSTRACT Vulvovaginal candidiasis is a common mucosal infection caused by opportunistic yeasts of the Candida genus. In this study, we isolated and identified the yeast species in the vagina of patients treated in the gynecology clinic and tested in vitro activities of fluconazole and itraconazole against 227 clinical yeast isolates by the NCCLS microdilution method. C. albicans (87.6%) was the most frequently identified species followed by C. glabrata (6.2%) and C. krusei (2.2%). Almost thirteen percent of yeast strains were resistant to fluconazole and 18.5% were resistant to itraconazole. Cross-resistance analyses of C. albicans isolates revealed that fluconazole resistance and itraconazole resistance were also associated with decreased susceptibilities to other azole derivatives mainly to ketoconazole and miconazole. At the same time no cross-resistance to polyene antibiotics amphotericin B and nystatin was observed. These results support the notion that antifungal agents used to treat vaginitis may be contributing to the drug resistance problem by promoting cross-resistance to a range of clinically used antifungals.

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Available from: Julius Subík, Mar 05, 2014
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    • "Susceptibilities of clinical isolates to FLU and ITR were determined by the broth microdilution method recommended by the National Committee for Clinical Laboratory Standards , as described previously [12] [19]. Susceptibilities of isolates to 5FC, 4-NQO and TER were determined by agar broth dilution method using minimal YNB medium buffered to pH 7 (for 5FC and 4-NQO) [19] or pH 6 (for TER) [20]. "
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    • "Sabatelli et al. [25] detected 6.4% resistance to fluconazole in 6,595 Candida isolates. Skrodeniene et al. [26] found 14 (15.1%) of 93 C. albicans strains resistant to fluconazole, while Sojakova et al. [27] reported fluconazole resistance as 13% in 227 Candida isolates. The rates of resistance are reported in a quite wide range in the studies carried out in our country as well. "
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    The Scientific World Journal 10/2013; 2013:236903. DOI:10.1155/2013/236903 · 1.73 Impact Factor
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    • "Candida glabrata is an opportunistic human pathogen responsible for candidaemia. Behind C. albicans, this haploid yeast is considered to be the second most commonly isolated Candida species from both bloodstream (Pfaller et al., 1999) and vaginal infections (Sojakova et al., 2004). It is evolutionarily more closely related to Saccharomyces cerevisiae than C. albicans. "
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