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Identification of Metabolites in Plasma and Urine of Uruguayan Propolis-Treated Rats

Laboratory of Functional Food Science and COE Program in the 21st Century, School of Food and Nutritional Sciences, University of Shizuoka, 52-1 Yada, Shizuoka 422-8526, Japan.
Journal of Agricultural and Food Chemistry (Impact Factor: 3.11). 06/2004; 52(10):3083-8. DOI: 10.1021/jf0353234
Source: PubMed

ABSTRACT Propolis is a resinous substance collected by honeybees from various plant sources. It is extensively used in food and beverages to improve health and prevent diseases such as heart disease, diabetes, and cancer. To investigate the absorption and metabolism of the components in propolis, in the present study, we administered ethanol extracts of Uruguayan propolis (poplar type propolis) orally to rats and analyzed their plasma and urine by high-performance liquid chromatography with photodiode array and mass spectrometric detection. After deconjugation of the components by beta-glucuronidase/sulfatase treatment of the specimen, pinobanksin 5-methyl ether, pinobanksin, kaempferol, chrysin, pinocembrin, and galangin were detected in plasma of rats orally administered propolis. These compounds were detected also in urine after beta-glucuronidase/sulfatase treatment. Furthermore, pinobanksin 5-methyl ether, pinobanksin, chrysin, pinocembrin, and galangin were present in the urine also in free form. These results suggest that flavonoids in propolis are metabolized and circulate in the body after oral administration of propolis.

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    • "That is, introducing the genes for biosynthesis of pinocembrin could increase natural crop resistance to herbivory, and/or produce high-yielding cultivars as a source of natural botanical insecticides. Pinocembrin can be extracted from numerous plants or from propolis (Kumazawa et al. 2004), and can be synthesized easily using established methods because of its molecular simplicity. Compared with commercial synthetic insecticides, pinocembrin may represent a more environmentally friendly insecticide, and its potential availability makes it a useful alternative. "
    Journal of Pest Science 01/2015; DOI:10.1007/s10340-014-0641-z · 2.66 Impact Factor
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    • "ompounds ( Fig . 4 ) . PIN is one of the major components of propolis . Collected from various natural plant sources , propolis is a resinous substance exten - sively eaten to improve health and to prevent diseases such as heart disease , diabetes , and cancer both as a dietary sup - plement and in applications within the pharmaceutical industry ( Kumazawa et al . , 2004 ) . Recently , much attention has been paid to PIN because of its benefits for human health due to anti - inflammatory , antioxidant , anti - thrombotic , antimicrobial , anti - allergic , hepatoprotective , anti - viral , cancer chemopreven - tive , and anti - asthmatic activities ( Hwang et al . , 2003 ; Sala et al . , 2003 ) . Howeve"
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    ABSTRACT: The increased expression and cross-linking activity of tissue transglutaminase (tTG) have been demonstrated in acute liver injury and fibrosis. We focused on the molecular mechanisms that contribute to ethanol-induced tTG expression and investigated the efficacy of propolis components in preventing both the tTG expression in vitro and fibrogenesis in vivo. We demonstrate herein that both ERK1/2 and PI3K/Akt pathways can regulate the effects of ethanol on NF-kappaB-dependent transcription and these signaling pathways may be involved in activation of ethanol-mediated tTG expression. We also found that administration of pinocembrin (PIN), one of the major components of propolis, inhibited tTG activation and significantly prevented the development of thioacetamide (TAA)-induced liver cirrhosis. The present study suggests that tTG may be an important member of the cascade of factors necessary for ethanol-induced liver fibrogenesis and PIN could serve as an anti-fibrogenic agent.
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    ABSTRACT: Abstract: Monosex tilapia is desirable in aquaculture to control reproduction and produce the gender with faster growth characteristics. Phytochemicals are present in many plants and have many reported biological properties. Here we explore the use of selected phytochemicals as potential in vivo inhibitors of aromatase and as antagonist in nuclear estrogen receptors in gonad germ cells, in order to modulate the gonad differentiation process in sexually undifferentiated Nile tilapia (Oreochromis niloticus). In a first trial, diets were supplemented with genistein (500 mg/kg) and quercetin (10g/kg) along with the androgenic synthetic hormone 17[alpha]-methyltestosterone (MT) (60 mg/kg), we evaluated the in vivo response towards masculinization in genetically all-female Nile tilapia. In a second trail, experimental diets with caffeic acid (500 mg/kg), chrysin (500 mg/kg), daidzein (500 mg/kg) including MT (60 mg/kg), along with the steroidal aromatase inhibitor 1,4,6-androstatrien-3-17-dione (ATD) (150 mg/kg), and spironolactone (500 mg/kg), were administered to all-female tilapia to assess the response in final phenotypic sex of the gonad. In this trial, phytochemicals and spironolactone were also administered to all-male tilapia. A control diet, free of chemicals was included in all trials. Our results indicate that the phenotypic sex is not affected by the inclusion of phytochemicals at supplemented levels in the diet. MT and ATD induced masculinization in both feeding trials. Final male ratio with MT was 86 and 100% for experiments 1 and 2 respectively, ATD induced a 50% masculinization. Spironolactone did not affect the phenotypic gender of tilapia. Survival and growth was not different across treatments in experiment 1 and all-male fish in experiment 2. In all-female experiment 2, treatment groups for MT and ATD were significantly smaller (p<0.05). Phytochemical absorption rates were validated with HPLC methods, after adaptation of extraction procedures and chromatographic conditions that allowed estimating concentrations in whole body after administration for either 6 or 8 weeks. Antioxidant biological activity of phytochemicals (quercetin) was also under study; here we explored the possible cumulative effect with ascorbic acid on experimental fish after acute UV-irradiation exposure in order to reduce ascorbic acid depletion in skin tissue, our results indicate no interaction between both antioxidants. 711.00 kB Title from first page of PDF file. Thesis (Ph. D.)--Ohio State University, 2005. Includes bibliographical references (p. 125-144). Available online via OhioLINK's ETD Center System requirements: World Wide Web browser and PDF viewer.
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