Epidermolysis bullosa acquisita sera react with distinct epitopes on the NC1 and NC2 domains of type VII collagen: study using immunoblotting of domain-specific recombinant proteins and postembedding immunoelectron microscopy
ABSTRACT The sera of epidermolysis bullosa acquisita (EBA) react with type VII collagen, a major component of anchoring fibrils, in which the major epitopes have been considered to be present in the N-terminal noncollagenous (NC) 1 domain.
To determine whether there are also epitopes in the C-terminal NC2 domain, and to determine their ultrastructural localization.
Immunoblotting using recombinant proteins of the NC1 and NC2 domains of type VII collagen, and postembedding immunoelectron microscopy.
Twenty of 28 EBA sera tested reacted with the NC1 domain and eight sera reacted with the NC2 domain. The sera that reacted with the NC1 domain showed immunoreactivity within the lamina densa and the sera that reacted with the NC2 domain showed immunoreactivity in the dermis 300-360 nm below the lamina densa.
This study clearly identified the presence of epitopes in the NC2 domain, and showed that the epitope in the NC1 domain is present in the lamina densa and that the epitope in the NC2 domain is in the dermis below the lamina densa.
- [Show abstract] [Hide abstract]
ABSTRACT: The problem of designing realistic decentralized controllers to solve the robust decentralized servomechanism problem  is considered in this paper. In particular, it is desired to find a decentralized controller for a plant to solve the robust servomechanism problem so that closed loop stability and asymptotic regulation occur, and also so that other desirable properties of the controlled system, such as fast response, low-interaction, integrity, tolerance to plant variations, constraints on gain magnitudes etc. occur. The method of design is based on extending the centralized design method of ,  as is described in . It is the purpose of this paper to outline some case studies of the design method when applied to plants varying from 2nd to 9th order with controllers of various complexity.Decision and Control, 1982 21st IEEE Conference on; 01/1983
Conference Paper: Estimation of velocity, acceleration and disparity in time sequences[Show abstract] [Hide abstract]
ABSTRACT: The paper presents a general framework for the analysis of time sequences. Features extracted include speed, acceleration and disparity/depth. The method uses spatio-temporal filtering in a hierarchical structure. Synthetic and real world examples are included.< >Visual Motion, 1991., Proceedings of the IEEE Workshop on; 11/1991
- [Show abstract] [Hide abstract]
ABSTRACT: A 75-year-old Japanese male visited us with bullous eruptions on the extremities. Physical examination revealed large bullae on the hands, lower legs and feet. The oral mucosa was also involved. Histology disclosed subepidermal blister with inflammatory cell infiltrates in the dermis. Direct immunofluorescence showed deposits of IgG and IgA at the cutaneous basement membrane zone. Indirect immunofluorescence on 1 M NaCl-split human skin sections demonstrated that the patient's IgG antibodies reacted with the dermal side of the split, while IgA antibodies reacted with the epidermal side. Immunoblotting showed that the patient's serum reacted with the NC1 domain of type VII collagen (290-kDa epidermolysis bullosa acquisita antigen) as well as the 120-kDa linear IgA bullous dermatosis antigen, LAD-1. Systemic prednisolone resulted in a favorable response. From the clinicopathological findings, the present case is not consistent with either epidermolysis bullosa acquisita or IgA bullous dermatosis. Therefore, we regarded the case as mixed bullous disease of epidermolysis bullosa acquisita and linear IgA bullous dermatosis. Such a case has not been previously reported.Dermatology 02/2005; 211(2):146-8. DOI:10.1159/000086445 · 1.69 Impact Factor