Epidermolysis bullosa acquisita sera react with distinct epitopes on the NC1 and NC2 domains of type VII collagen: study using immunoblotting of domain-specific recombinant proteins and postembedding immunoelectron microscopy
ABSTRACT The sera of epidermolysis bullosa acquisita (EBA) react with type VII collagen, a major component of anchoring fibrils, in which the major epitopes have been considered to be present in the N-terminal noncollagenous (NC) 1 domain.
To determine whether there are also epitopes in the C-terminal NC2 domain, and to determine their ultrastructural localization.
Immunoblotting using recombinant proteins of the NC1 and NC2 domains of type VII collagen, and postembedding immunoelectron microscopy.
Twenty of 28 EBA sera tested reacted with the NC1 domain and eight sera reacted with the NC2 domain. The sera that reacted with the NC1 domain showed immunoreactivity within the lamina densa and the sera that reacted with the NC2 domain showed immunoreactivity in the dermis 300-360 nm below the lamina densa.
This study clearly identified the presence of epitopes in the NC2 domain, and showed that the epitope in the NC1 domain is present in the lamina densa and that the epitope in the NC2 domain is in the dermis below the lamina densa.
Article: Real time counter machines[Show abstract] [Hide abstract]
ABSTRACT: An automaton called the balloon automaton is defined. The balloon automaton comes in four varieties, depending on whether the device is deterministic or nondeterministic, and whether the input head can move in one or two directions. Subsets of the balloon automata of each variety, called closed classes are defined. Almost all the known types of automata are equivalent to some closed class of balloon automata. Properties of closed classes are given. For example, whatever the variety, the languages accepted by a closed class are closed under intersection with a regular set. For a given organization of storage, closed classes of the four varieties can be defined. These four classes are said to form a family. A class may be recursive or not, and the emptiness problem may be solvable or unsolvable. Some surprising relationships exist between the recursiveness and solvability of emptiness for the classes in a family.
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ABSTRACT: Compression filters with bandwidths up to 1000 MHz have application in high-resolution RADAR system and rapid-scan receiver systems. A technique is presented for realizing a microwave linear delay (quadratic phase) versus frequency compression filter with sufficient delay accuracy to make compression ratios of up to 1000 to 1 feasible. The dispersive element in the compression filter is a silver tape with its broad side placed perpendicularly between the ground planes (instead of parallel, as in conventional stripline). The tape is folded back and forth upon itself in such a way that substantial coupling takes place between adjacent turns of the tape. A computer program has been written to determine the dimensions of the tape to achieve a linear delay versus frequency characteristic. A folded tape compression filter was constructed with a differential delay of 1.2 /spl mu/s over a bandwidth of 600 MHz centered at 1350 MHz giving a compression factor of 720 to 1. This filter was constructed in four identical sections, each section of which had a differential delay of 0.3 /spl mu/s over the same bandwidth as the complete filter. The entire filter (four sections) occupies a volume about 16 by 4 by 5 inches. Measurement data are presented which illustrate that the desired accurate delay characteristic was realized to within the /spl plusmn/ 1 ns measurement uncertainty.IEEE Transactions on Microwave Theory and Techniques 01/1968; DOI:10.1109/TMTT.1967.1126567 · 2.94 Impact Factor
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ABSTRACT: The problem of designing realistic decentralized controllers to solve the robust decentralized servomechanism problem  is considered in this paper. In particular, it is desired to find a decentralized controller for a plant to solve the robust servomechanism problem so that closed loop stability and asymptotic regulation occur, and also so that other desirable properties of the controlled system, such as fast response, low-interaction, integrity, tolerance to plant variations, constraints on gain magnitudes etc. occur. The method of design is based on extending the centralized design method of ,  as is described in . It is the purpose of this paper to outline some case studies of the design method when applied to plants varying from 2nd to 9th order with controllers of various complexity.Decision and Control, 1982 21st IEEE Conference on; 01/1983