Article

Automated Flow Cytometric Analysis of Blood Cells in Cerebrospinal Fluid Analytic Performance

St Olaf's University Hospital, Trondheim, Norway.
American Journal of Clinical Pathology (Impact Factor: 3.01). 06/2004; 121(5):690-700. DOI: 10.1309/EKFW-9E3L-LFXE-15X9
Source: PubMed

ABSTRACT We compared the performance of an automated method for obtaining RBC and WBC counts and WBC differential counts in cerebrospinal fluid (CSF) samples with the reference manual method. Results from 325 samples from 10 worldwide clinical sites were used to demonstrate the accuracy, precision, and linearity of the method. Accuracy statistics for absolute cell counts showed a high correlation between methods, with correlation coefficients for all reportable absolute counts greater than 0.9. Linearity results demonstrated that the method provides accurate results throughout the reportable ranges, including clinical decision points for WBCs of 0 to 10/microL. Interassay precision and intra-assay precision for the ADVIA 120 (Bayer HealthCare, Tarrytown, NY) method were acceptable at all levels. The ADVIA 120 CSF Assay enumerates and differentiates cells via flow cytometry in a minimally diluted sample, improving the analysis of typically hypocellular CSF samples. Study results demonstrate that the automated ADVIA 120 CSF Assay is an acceptable alternative to the labor-intensive manual method.

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    • "The automated counting is faster, easier as it does not require pretreatment of the samples and analytically more precise than manual counting and does not require a particularly qualified personnel. Several studies previously reported on automated counting of synovial fluid (Salinas et al., 1997; de Jonge et al., 2004), CSF (Ziebig, 2000; Aune et al., 2004), pleural fluid (Conner et al., 2003; De Jonge et al., 2006), ascitic/peritoneal fluid (Angeloni et al., 2003), and body fluids in general (Aulesa et al., 2003; Kresie et al., 2005). To compare the microscopic method with the automated XE-5000 method, we initiated a study and analyzed one hundred and seventy four (n = 174) body fluid samples including 81 ascitic fluids, 32 CSF, 26 pleural fluids, 18 synovial fluids, 13 peritoneal fluids, and 4 other types. "
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    ABSTRACT: We evaluated the performance of the automated body fluid mode of the Sysmex XE-5000 series automated haematology analyzer and compared the performance of the automated method for obtaining white blood cell (WBC), red blood cell (RBC) counts and WBC differential counts with microscopic method. One hundred and seventy-four samples were analysed: 81 ascitic fluid, 32 cerebrospinal fluid (CSF), 26 pleural fluid (PF), 18 synovial fluid (SF), 13 peritoneal fluid (PeF) and 4 other types. The agreement between the automated method and the manual reference showed high correlation, with Pearson correlation coefficients greater than 0.9 for all types of body fluids. We also demonstrate that the automated body fluid analysis on the XE-5000 is an acceptable alternative to the microscopic reference method as far as ascitic fluid, peritoneal dialysis fluid, SF or PF are concerned. Conversely, results for body fluid samples from oncology patients with leukaemia or tumours showed significant differences between both methods, as XE-5000 counted blast cells and neoplastic cells in mononuclear cell count. XE-5000 could represent an attractive method for the automated analysis of WBC, RBC, mononuclear cell count (MNC) and polymorphonuclear (PMN) cells of most body fluids. However, CSFs from patients with leukaemia or lymphoma should be processed with the microscopic reference method in order to detect abnormal leukaemic cells.
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