Automated Flow Cytometric Analysis of Blood Cells in Cerebrospinal Fluid Analytic Performance

St Olaf's University Hospital, Trondheim, Norway.
American Journal of Clinical Pathology (Impact Factor: 3.01). 06/2004; 121(5):690-700. DOI: 10.1309/EKFW-9E3L-LFXE-15X9
Source: PubMed

ABSTRACT We compared the performance of an automated method for obtaining RBC and WBC counts and WBC differential counts in cerebrospinal fluid (CSF) samples with the reference manual method. Results from 325 samples from 10 worldwide clinical sites were used to demonstrate the accuracy, precision, and linearity of the method. Accuracy statistics for absolute cell counts showed a high correlation between methods, with correlation coefficients for all reportable absolute counts greater than 0.9. Linearity results demonstrated that the method provides accurate results throughout the reportable ranges, including clinical decision points for WBCs of 0 to 10/microL. Interassay precision and intra-assay precision for the ADVIA 120 (Bayer HealthCare, Tarrytown, NY) method were acceptable at all levels. The ADVIA 120 CSF Assay enumerates and differentiates cells via flow cytometry in a minimally diluted sample, improving the analysis of typically hypocellular CSF samples. Study results demonstrate that the automated ADVIA 120 CSF Assay is an acceptable alternative to the labor-intensive manual method.

1 Follower
  • Source
    • "The automated counting is faster, easier as it does not require pretreatment of the samples and analytically more precise than manual counting and does not require a particularly qualified personnel. Several studies previously reported on automated counting of synovial fluid (Salinas et al., 1997; de Jonge et al., 2004), CSF (Ziebig, 2000; Aune et al., 2004), pleural fluid (Conner et al., 2003; De Jonge et al., 2006), ascitic/peritoneal fluid (Angeloni et al., 2003), and body fluids in general (Aulesa et al., 2003; Kresie et al., 2005). To compare the microscopic method with the automated XE-5000 method, we initiated a study and analyzed one hundred and seventy four (n = 174) body fluid samples including 81 ascitic fluids, 32 CSF, 26 pleural fluids, 18 synovial fluids, 13 peritoneal fluids, and 4 other types. "
    [Show abstract] [Hide abstract]
    ABSTRACT: We evaluated the performance of the automated body fluid mode of the Sysmex XE-5000 series automated haematology analyzer and compared the performance of the automated method for obtaining white blood cell (WBC), red blood cell (RBC) counts and WBC differential counts with microscopic method. One hundred and seventy-four samples were analysed: 81 ascitic fluid, 32 cerebrospinal fluid (CSF), 26 pleural fluid (PF), 18 synovial fluid (SF), 13 peritoneal fluid (PeF) and 4 other types. The agreement between the automated method and the manual reference showed high correlation, with Pearson correlation coefficients greater than 0.9 for all types of body fluids. We also demonstrate that the automated body fluid analysis on the XE-5000 is an acceptable alternative to the microscopic reference method as far as ascitic fluid, peritoneal dialysis fluid, SF or PF are concerned. Conversely, results for body fluid samples from oncology patients with leukaemia or tumours showed significant differences between both methods, as XE-5000 counted blast cells and neoplastic cells in mononuclear cell count. XE-5000 could represent an attractive method for the automated analysis of WBC, RBC, mononuclear cell count (MNC) and polymorphonuclear (PMN) cells of most body fluids. However, CSFs from patients with leukaemia or lymphoma should be processed with the microscopic reference method in order to detect abnormal leukaemic cells.
    International journal of laboratory hematology 03/2010; 32(5):539-47. DOI:10.1111/j.1751-553X.2010.01220.x · 1.87 Impact Factor
  • Source
    • "Automated urine microscopy analyzers do not allow sensitive detection of bacteria for CSF applications [8] [9]. The application originally conceived for urinalysis flow cytometers for CSF applications heralded a new era [1] [3] [4]. Sysmex UF-100 (TOA Medical Electronics, Kobe, Japan), originally intended for urinalysis purposes, shows an apparent " noise " in the bacterial channel in microbiologically confirmed sterile CSF and continuous ambulatory peritoneal dialysis (CAPD) fluids [1]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Caution is of paramount importance in the interpretation of flow cytometric white blood cell counts in specimens with a high lymphocyte percentage as it is in the interpretation of bacterial counts in hemorrhagic and ventricular drainage CSF specimens. Recently, flow cytometry using a semiconductor laser along with forward and sideward scatter detection and also a dedicated bacterial channel has been developed (Sysmex UF-1000i). We explored the possible applications of this novel approach in the differential diagnosis of meningitis. Flow cytometry, microscopy and biochemical data of 161 CSF samples were analyzed. Microbiological analysis was performed in 53 suspected cases of meningitis. Good agreement for leukocytes was obtained between UF-1000i (rho=0.614) and UF-100 (rho=0.582) and microscopy and between both flow cytometers (rho=0.734). Lymphocytes were correctly classified as WBC by UF-1000i. Bacterial count on UF-1000i showed to be reliable in hemorrhagic samples and in samples collected by ventricular drainage for which interference by blood platelets and cell debris forms a known caveat on UF-100. Bacterial background signal in sterile CSF samples was absent on UF-1000i. One case of Cryptococcus neoformans meningitis, missed by UF-100 was properly detected by UF-1000i. Sysmex UF-1000i CSF flow cytometer offers the clinician an improved aid in directing the differential diagnosis of meningitis towards viral, bacterial or fungal causes.
    Clinica Chimica Acta 07/2008; 392(1-2):30-3. DOI:10.1016/j.cca.2008.02.020 · 2.82 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Pegasus is an object-oriented multidatabase system being developed at Hewlett-Packard Laboratories. The goal of the system is to provide facilities for multidatabase applications for accessing and manipulating multiple autonomous heterogeneous object-oriented, relational and other databases. This paper gives an overview of the architecture, describes the common object model and gives the justification for adapting the model for a multidatabase system. It describes the database environment of the system, discusses how to manage schemas consisting of types and functions from multiple databases. It briefly describes the features of the data language and gives an example. It discusses a method for dealing with object identification issues in a multidatabase system, reviews schema integration issues and briefly mentions schema mapping to relational models
    Interoperability in Multidatabase Systems, 1991. IMS '91. Proceedings., First International Workshop on; 05/1991
Show more


Available from