Leptin Receptor Signaling in POMC Neurons Is Required for Normal Body Weight Homeostasis

Department of Medicine, Division of Endocrinology, Beth Israel Deaconess Medical Center, Harvard Medical School, 99 Brookline Avenue RN, Boston, MA 02215, USA.
Neuron (Impact Factor: 15.05). 07/2004; 42(6):983-91. DOI: 10.1016/j.neuron.2004.06.004
Source: PubMed


Neuroanatomical and electrophysiological studies have shown that hypothalamic POMC neurons are targets of the adipostatic hormone leptin. However, the physiological relevance of leptin signaling in these neurons has not yet been directly tested. Here, using the Cre/loxP system, we critically test the functional importance of leptin action on POMC neurons by deleting leptin receptors specifically from these cells in mice. Mice lacking leptin signaling in POMC neurons are mildly obese, hyperleptinemic, and have altered expression of hypothalamic neuropeptides. In summary, leptin receptors on POMC neurons are required but not solely responsible for leptin's regulation of body weight homeostasis.

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Available from: Robert A McGovern, Aug 15, 2014
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    • "backbone of RP23-352G18 BAC DNA were replaced via homologous recombination [25] by an ampicillin resistance gene cassette generated via PCR amplification of a PGEM-T-Easy vector template with the primer sets: 5 0 GATAAACTACCGCATTAAAGCTTATCGATGATAAGCTGTCAAACATGAG- AATTGATCCGGATATATGAGTAAACTTGGTCTGAC and 5 0 GTTAACCGGGCTGCATCCGATGCAAGTGTGTCGCTGTCGACGGTGACC- CTATAGTCGAGGCGGTATTTTCTCCTTACGCATC. The modified RP23- 352G18 BAC DNA was then purified and microinjected in its circular state into pronuclei of fertilized embryos of C57Bl/6J mice using standard methods [24]. By genotyping of tail biopsies using the following primer sets 5 0 TTGCTCTTGCGGGAAGCCCG and 5 0 GATACCGAGGGCGCCGTTCG (specific to the pBACe3.6 "
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