Article

Crystallization and preliminary X-ray analysis of a C-terminal TonB fragment from Escherichia coli.

Department of Biology, University of Konstanz, 78457 Konstanz, Germany.
Acta Crystallographica Section D Biological Crystallography (impact factor: 12.62). 08/2004; 60(Pt 7):1281-3. DOI:10.1107/S0907444904009722 pp.1281-3
Source: PubMed

ABSTRACT The TonB protein located in the cell wall of Gram-negative bacteria mediates the proton motive force from the cytoplasmic membrane to specific outer membrane transporters. A C-terminal fragment of TonB from Escherichia coli consisting of amino-acid residues 147-239 (TonB-92) has been purified and crystallized. Crystals grew in space group P2(1) to dimensions of about 1.0 x 0.12 x 0.12 mm. A native data set has been obtained to 1.09 A resolution.

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Keywords

amino-acid residues 147-239
 
cytoplasmic membrane
 
Escherichia coli
 
Gram-negative bacteria mediates
 
proton motive force
 
specific outer membrane transporters