Aptamer-based sensor arrays for the detection and quantitation of proteins.

Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, Texas 78712, USA.
Analytical Chemistry (Impact Factor: 5.7). 08/2004; 76(14):4066-75. DOI: 10.1021/ac049858n
Source: PubMed

ABSTRACT Aptamer biosensors have been immobilized on beads, introduced into micromachined chips on the electronic tongue sensor array, and used for the detection and quantitation of proteins. Aptamer chips could detect proteins in both capture and sandwich assay formats. Unlike most protein-based arrays, the aptamer chips could be stripped and reused multiple times. The aptamer chips proved to be useful for screening aptamers from in vitro selection experiments and for sensitively quantitating the biothreat agent ricin.

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