Article

Aptamer-Based Sensor Arrays for the Detection and Quantitation of Proteins

Department of Electrical & Computer Engineering, University of Texas at Austin, Austin, Texas, United States
Analytical Chemistry (Impact Factor: 5.83). 08/2004; 76(14):4066-75. DOI: 10.1021/ac049858n
Source: PubMed

ABSTRACT Aptamer biosensors have been immobilized on beads, introduced into micromachined chips on the electronic tongue sensor array, and used for the detection and quantitation of proteins. Aptamer chips could detect proteins in both capture and sandwich assay formats. Unlike most protein-based arrays, the aptamer chips could be stripped and reused multiple times. The aptamer chips proved to be useful for screening aptamers from in vitro selection experiments and for sensitively quantitating the biothreat agent ricin.

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    • "3.1. Experimental principle of the aptamer-based EWA biosensor Aptamers exhibit high target-binding affinity because their binding function is largely dependent upon stable secondary structure interactions (Kirby et al., 2004; Zhou et al., 2010); however, it is challengeable to keep the restoration to the original DNA probe form after repeated uses (Liu and Tan, 1999). Inspired by the immunosensors undergoing the antibody–antigen recognition which feature with highly reusability of up to 300 times of regenerated test cycles (Rodriguez-Mozaz et al., 2005; Zhou et al., 2014), we describe here a novel aptamer-based EWA biosensing strategy as shown in Scheme 1. "
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    • "Be‐ cause of their high thermostability when compared with antibodies, aptamers have potential applications in analytical devices, including biosensors, and as therapeutic agents [38]. Assays for protein identification and quantitation were developed and ap‐ plied to ricin detection [39] [40]. A multiplex aptamer microarray was generated by print‐ ing an anti-ricin RNA aptamer onto either streptavidin (SA)-or neutravidin (NA)-coated glass slides. "
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    • "c o m / l o c a t e / t ox i c on ricin in food and water. Conventional analytical methods including radioimmunoassay (Godal et al., 1981), enzymelinked immunosorbent assay (ELISA) (Poli et al., 1994), fluorescence-based fiber optic immunoassay (Narang et al., 1997) aptamer microarrays (Kirby et al., 2004), masssensitive biosensor (Tran et al., 2008), microelectrochemical biosensors (Guglielmo-Viret and Thullier, 2007) and electrochemiluminescent (ECL) assay (Garber and O'Brien, 2008) are effective for ricin detection. However these analytical methods are time-consuming and therefore not suitable for biodefense. "
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