Molecular imaging of cardiovascular gene products

Department of Medicine, Stanford University, Palo Alto, California, United States
Journal of Nuclear Cardiology (Impact Factor: 2.65). 08/2004; 11(4):491-505. DOI: 10.1016/j.nuclcard.2004.04.004
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Available from: Joseph C Wu, Mar 23, 2015
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    • "To accurately assess stem cell survival in the living subject, it is imperative that a non-invasive imaging strategy is used. Novel developments in non-invasive imaging have allowed us to study transgene expression and the biology of cell therapy, using imaging modalities such as bioluminescence imaging (BLI) (Contag and Ross 2002; Negrin and Contag 2006; Shah et al. 2004; Wu et al. 2004). Our laboratory has previously demonstrated that cell survival can be monitored longitudinally (Rodriguez-Porcel et al. 2005). "
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    ABSTRACT: To investigate the mechanisms underlying the beneficial effect of hypoxia preconditioning (HPC) on mesenchymal stromal cells (MSCs) and optimize novel non-invasive methods to assess the effect of biological interventions aimed to increased cell survival. MSCs from rat femur, with or without HPC, were exposed to hypoxic conditions in cell culture (1% O(2) for 24h) and cell survival (by the LDH release assay and Annexin-V staining) was measured. Oxidant status (conversion of dichloro-fluorescein-DCF- and dihydro-ethidium-DHE-, protein expression of oxidant enzymes) was characterized, together with the mobility pattern of cells under stress. Furthermore, cell survival was assessed non-invasively using state-of-the-art molecular imaging. Compared to controls, Hypoxia resulted in increased expression of the oxidative stress enzyme NAD(P)H oxidase (subunit 67(phox): 0.05 ± 0.01AU and 0.48 ± 0.02AU, respectively, p<0.05) and in the amount of ROS (DCF: 13 ±1 and 42 ± 3 RFU/μg protein, respectively, p<0.05) which led to a decrease in stem cell viability. Hypoxia preconditioning preserved cell biology, as evidenced by preservation of oxidant status (16 ± 1 RFU/μg protein, p<0.05 vs. hypoxia), and cell viability. Most importantly, the beneficial effect of HPC can be assessed non-invasively using molecular imaging. HPC preserves cell viability and function, in part through preservation of oxidant status, and its effects can be assessed using state-of-the-art molecular imaging. Understanding of the mechanisms underlying the fate of stem cells will be critical for the advancement of the field of stem cell therapy.
    Life sciences 11/2010; 88(1-2):65-73. DOI:10.1016/j.lfs.2010.10.023 · 2.30 Impact Factor
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    • "A number of methods are available to track stem cells by molecular imaging. In general, there are two methods to label the cells: (1) direct labeling method, which physically introduce marker(s) into the cells before transplant; (2) indirect labeling method, which genetically introduce reporter gene(s) into the cells before transplant [Wu et al., 2004] "
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    ABSTRACT: Using endothelial cells for therapeutic angiogenesis/vasculogenesis of ischemia diseases has led to exploring human embryonic stem cells (hESCs) as a potentially unlimited source for endothelial progenitor cells. With their capacity for self-renewal and pluripotency, hESCs and their derived endothelial cells (hESC-ECs) may be more advantageous than other endothelial cells obtained from diseased populations. However, hESC-ECs' poor differentiation efficiency and poorly characterized in vivo function after transplantation present significant challenges for their future clinical application. This review will focus on the differentiation pathways of hESCs and their therapeutic potential for vascular diseases, as well as the monitoring of transplanted cells' fate via molecular imaging. Finally, cell enhancement strategies to improve the engraftment efficiency of hESC-ECs will be discussed.
    Journal of Cellular Biochemistry 02/2009; 106(2):194-9. DOI:10.1002/jcb.22003 · 3.37 Impact Factor