Ethanol Production Using Concentrated Oak Wood Hydrolysates and Methods to Detoxify

Biomass Research Team, Korea Institute of Energy Research, P.O. Box 5, Taedok Science Town, Taejon 305-343, Korea.
Applied Biochemistry and Biotechnology (Impact Factor: 1.74). 02/1999; 77-79(1-3):547-59. DOI: 10.1385/ABAB:78:1-3:547
Source: PubMed


Ethanol production from concentrated oak wood hydrolysate was carried out to obtain a high ethanol concentration and a high ethanol yield. The effect of added inhibitory compounds, which are typically produced in the pretreatment step of steam-explosion on ethanol fermentation, was also examined. p-Hydroxybenzoic aldehyde, a lignin-degradation product, was the most inhibitory compound tested in this study. Compounds with additional methyl groups had reduced toxicity and the aromatic acids were less toxic than the corresponding aldehydes. The lignin-degradation products were more inhibitory than the sugar-derived products, such as furfural and 5-hydroxymethylfurfural (HMF). Adaptation of yeast cells to the wood hydrolysate and detoxification methods, such as using charcoal and overlime, had some beneficial effects on ethanol production using the concentrated wood hydrolysate. After treatment with charcoal and low-temperature sterilization, the yeast cells could utilize the concentrated wood hydrolysate with 170 as well as 140 g/L glucose, and produce 69.9 and 74.2 g/L ethanol, respectively, with a yield of 0.46-0.48 g ethanol/g glucose. In contrast, the cells could not completely utilize untreated wood hydrolysate with 100 g/L glucose. Low-temperature sterilization, with or without charcoal treatment, was very effective for ethanol production when highly concentrated wood hydrolysates were used. Low-temperature sterilization has advantages over traditional detoxification methods, such as using overlime, ion exchange, and charcoal, because of the reduction in the total cost of ethanol production.

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    • "furfural, acetic acid and lignin derivatives (Carvalho et al. 2004a; Silva et al. 2005). These inhibitory compounds affect the ability of yeast to ferment sugars in hydrolysates, and thus to improve microbial production of ethanol from lignocellulosic hydrolysates, different detoxification methods like pH adjustment (Eken-Saraçoglu and Arslan 2000; Martinez et al. 2001), activated charcoal adsorption (Lee et al. 1999; Mussatto and Roberto 2001), and ion exchange resin adsorption (Carvalho et al. 2004b; Villarreal et al. 2006) have been proposed. "
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    • "d Assuming 7% (w/v) ethanol produced from fermentation in a CBP system (Lynd et al., 2008). e Lee et al. (1999). f Larsson et al. (2000). "
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    • "Very often hydrolysates containing toxic substances have to be purified before they can be used as fermentation media . To overcome the inhibitory effect of the toxic compounds during fermentation by yeasts, several types of treatment have been employed, including microorganism adaptation [2] [8], evaporation [9] [10], overtitration and overliming [11] [12], and adsorption on ion-exchange resins [13] [14], or on activated charcoal [15] [16]. Combinations of these treatments are also reported in the literature [10] [17] [18] "
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