Topical β‐carotene is converted to retinyl esters in human skin ex vivo and mouse skin in vivo

Department of Dermatology, University Hospital, Geneva, Switzerland.
Experimental Dermatology (Impact Factor: 3.76). 10/2004; 13(9):558-61. DOI: 10.1111/j.0906-6705.2004.00194.x
Source: PubMed

ABSTRACT Human epidermis contains endogenous retinoids (retinol and retinyl esters) and carotenoids (mostly beta-carotene). Previous studies have shown that the enzymes involved in retinoid metabolism are present in human epidermis. There is still a controversy about the presence in the skin of the enzymes able to convert beta-carotene into vitamin A (retinol), although a recent study demonstrated the conversion of beta-carotene into retinol in human cultured epidermal cells. In this study, we addressed the question of the possible bioconversion of topical beta-carotene into vitamin A or derivatives by human and mouse skin. Surgically excised human abdominal skin was mounted on Franz perfusion chambers to assess the cutaneous penetration of topical beta-carotene as well as its metabolism, after a 24-h incubation period, whereas hairless mice received topical beta-carotene 24 h before assaying epidermal beta-carotene and retinoid concentrations. Epidermal retinoid and beta-carotene concentrations were determined by high-pressure liquid chromatography. Topical beta-carotene penetrated well into human and mouse epidermis and induced a 10-fold (human) and a threefold (mouse) increase of epidermal retinyl esters, which demonstrates that topical beta-carotene is converted into retinyl esters by human and mouse epidermis and thus appears as a precursor of epidermal vitamin A.

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    • "Also, more specialized acyltransferases exist in human keratinocytes to assure the storage of the important skin retinol through esterification into retinyl esters. This step is catalyzed by two enzymes, acyl-CoA: retinol acyltransferase and lecithin: retinol acyltransferase ; their expression is modulated by the differentiation state of the keratinocytes (Antille et al. 2004; Törmä and Vahlquist 1990). "
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