The antioxidant potential of Sutherlandia frutescens

Department of Pharmacology, Faculty of Health Sciences, University of Pretoria, PO Box 2034, Pretoria 0001, South Africa.
Journal of Ethnopharmacology (Impact Factor: 3). 12/2004; 95(1):1-5. DOI: 10.1016/j.jep.2004.05.024
Source: PubMed

ABSTRACT One of the best-known multi-purpose medicinal plants in Southern Africa, Sutherlandia frutescens subsp. microphylla (family: Fabaceae/Leguminosa), is used for a wide range of conditions, including cancer, viral diseases and inflammatory conditions. Little scientific data has been documented on the mechanism by which Sutherlandia frutescens acts on the immune system. Phagocyte derived reactive oxygen species, such as hydrogen peroxide and superoxide radicals, are responsible for the pathogenesis of various inflammatory conditions. Anti-inflammatory properties of various medicinal-plant extracts have been explained, at least in part, by their antioxidant activities. We investigated the effects of a hot water extract of Sutherlandia frutescens on both luminol and lucigenin enhanced chemiluminescence of neutrophils stimulated with L-formyl-L-methionyl-L-leucyl-L-phenylalanine (FMLP) as well as its superoxide and hydrogen peroxide scavenging properties in a cell free system. The results indicate that Sutherlandia frutescens extract possesses superoxide as well as hydrogen peroxide scavenging activities at concentrations as low as 10 microg/ml, which could account for some of the anti-inflammatory properties that have been described.

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Available from: Duncan Cromarty, Sep 28, 2015
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    • "The product identity was confirmed using HPLC/ELSD and HPLC/ UV (Avula et al., 2010), which determined that the S. frutescens used in this study contained 3.3% (w/w) of sutherlandioside B, a specific biomarker of this medicinal plant (Avula et al., 2010; Fu et al., 2008). An aqueous extract of S. frutescens was prepared using the method described by Fernandes et al. (2004). Briefly, 10 g of finely ground S. frutescens was added to 250 mL of boiling water. "
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    ABSTRACT: Sutherlandia frutescens (L.) R. Br. is an indigenous plant of southern Africa that has been traditionally used for various cancers, infections, and inflammatory conditions. Our aim was to investigate the potential immuno-stimulatory activity of a polysaccharide-enriched fraction (SFPS) from a decoction of S. frutescens. RAW 264.7 cells (a murine macrophage cell line) were used to determine the activities of SFPS on macrophage function. The production of reactive oxygen species (ROS), nitric oxide (NO), and inflammatory cytokines were evaluated in the cells treated with or without SFPS. CLI-095, a toll-like receptor (TLR) 4-specific inhibitor, was used to identify whether or not SFPS exerts its effects through TLR4. An antagonist of endotoxin, polymyxin B, was used to evaluate whether endotoxin present in SFPS contributed to its immune-stimulatory activity. SFPS exhibited potent immune-stimulatory activity by macrophages. The production of ROS, NO, and tumor necrosis factor (TNF-α) were increased upon exposure to SFPS in a dose-dependent manner. All of these activities were completely blocked by co-treatment with CLI-095, but only partially diminished by polymyxin B. We demonstrate for the first time potent immune-stimulatory activity in a decoction prepared from S. frutescens. We believe that this immune stimulatory activity is due, in part, to the action of polysaccharides present in the decoction that acts by way of TLR4 receptors and the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling pathway. These findings provide a plausible mechanism through which we can understand some of the medicinal properties of S. frutescens. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.
    Journal of ethnopharmacology 06/2015; 172. DOI:10.1016/j.jep.2015.06.013 · 3.00 Impact Factor
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    • "The results provide further support that the two species are chemically similar and there is little chemical information that can be used to differentiate them. This result is also supported by previous reports where S. microphylla has been referred to as a taxonomic ally and morphological variant of S. frutescens (Fernandes et al., 2004; Smith and Myburgh, 2004; Van Wyk and Albrecht, 2008). An S-plot of the OPLS-DA model was used to identify compounds that may be used for the differentiation between the two species and the results obtained are shown in Fig. 5. "
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    ABSTRACT: Sutherlandia frutescens (Fabaceae) commonly known as cancer-bush, is a well-known traditional phytomedicine in South Africa used to treat a range of ailments. There is limited information available on the phytochemistry and chemical variation within and between the S. frutescens and Sutherlandia microphylla species complex. This paper aims to elucidate the chemical variation of phytoconstituents (other than the non-protein amino acids) between the two species S. frutescens and S. microphylla and also between the wild and cultivated varieties of S. frutescens. An UPLC–MS analysis in tandem with chemometric analysis has been performed to assess the metabolite content of aerial plant parts obtained from different populations. Principal component analysis (PCA) was performed to observe groupings and trends in the data matrix. An orthogonal partial least square discriminant analysis (OPLS-DA) was performed which resulted in clear groups between the two taxa. Several flavonoid and triterpenoid glycoside derivatives contribute to the quantitative chemotypic variation within and between the species as observed. The identification of these compounds using advanced chromatographic techniques (UPLC–MS) and chemometric analysis leads to a better understanding of the phytochemical variation of Sutherlandia which can aid in quality control of raw material, phytomedicines and commercial herbal products.
    Biochemical Systematics and Ecology 10/2014; 56:221–230. DOI:10.1016/j.bse.2014.06.009 · 0.97 Impact Factor
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    • "Sutherlandia frutescens (SF) has long been used as a traditional medicinal plant in southern Africa for treatment of cancer, as well as a variety of chronic ailments, and more recently, HIV/AIDS [1]–[3]. Limited studies suggest multiple actions of SF as a consequence of putative antioxidant and anti-inflammatory activities [4]–[8], including inhibition of phorbol ester-induced COX-2 expression in human breast epithelial cells and mouse skin [6], [7]. There are also indications that SF has neuroprotective effects, such as alleviating symptoms associated with stress [2] as well as convulsions and epilepsy [9]. "
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    ABSTRACT: Sutherlandia frutescens (L.) R.Br. (SF) is a medicinal plant indigenous to southern Africa and used in folk and contemporary remedies for stress, chronic diseases, cancer, and HIV/AIDS. While previous studies have focused on physiological effects of SF on cellular and systemic abnormalities associated with these diseases, little is known about its effects in the brain and immune cells in the central nervous system. Results of this study indicate that ethanol extracts of SF (SF-E) suppress NMDA-induced reactive oxygen species (ROS) production in neurons, and LPS- and IFNγ-induced ROS and nitric oxide (NO) production in microglial cells. SF-E's action on microglial cells appears to be mediated through inhibition of the IFNγ-induced p-ERK1/2 signaling pathway which is central to regulating a number of intracellular metabolic processes including enhancing STAT1α phosphorylation and filopodia formation. The involvement of SF in these pathways suggests the potential for novel therapeutics for stress and prevention, and/or treatment of HIV/AIDS as well as other inflammatory diseases in the brain.
    PLoS ONE 02/2014; 9(2):e89748. DOI:10.1371/journal.pone.0089748 · 3.23 Impact Factor
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