Article

Determination of bisphenol A in human breast milk by HPLC with column-switching and fluorescence detection.

Division of Analytical Research for Pharmacoinformatics, Department of Clinical Chemistry, Course of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.
Biomedical Chromatography (impact factor: 1.97). 11/2004; 18(8):501-7. DOI:10.1002/bmc.345 pp.501-7
Source: PubMed

ABSTRACT A highly sensitive HPLC method was developed for the determination of xenoestrogenic compound, bisphenol A (BPA) in human breast milk samples. After a two-step liquid-liquid extraction, BPA was derivatized with fluorescent labeling reagent, 4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride (DIB-Cl). The excess fluorescent reagent could be removed effectively using a column-switching system. The separation of DIB-BPA from endogenous materials in milk was carried out on two C(18) columns and fluorescence intensity was monitored at 475 nm with the excitation of 350 nm. A good linearity (r = 0.994) was observed of BPA in the concentration range of 0.2-5.0 ng mL(-1) in breast milk, and the detection limit was 0.11 ng mL(-1) at a signal-to-noise ratio of 3. Intra- and inter-day precision (RSD, %) were less than 8.7 and 10.4, respectively. Twenty-three breast milk samples of healthy lactating women were analyzed for the BPA concentration; the mean value was 0.61 +/- 0.20 ng mL(-1), with no correlation to the lipid content of milk samples.

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Keywords

4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride
 
bisphenol
 
BPA concentration
 
breast milk
 
breast milk samples
 
column-switching system
 
concentration range
 
detection limit
 
endogenous materials
 
excitation
 
fluorescent labeling reagent
 
healthy lactating women
 
human breast milk samples
 
mean value
 
milk samples
 
sensitive HPLC method
 
signal-to-noise ratio
 
two-step liquid-liquid extraction