Article

Time-lapse and cell ablation reveal the role of cell interactions in fly glia migration and proliferation.

Institut de Génétique et Biologie Moléculaire et Cellulaire, IGBMC/CNRS/ULP/INSERM - BP 10142, ILLKIRCH, C. U. de Strasbourg 67404, France.
Development (impact factor: 6.6). 11/2004; 131(20):5127-38. DOI:10.1242/dev.01398 pp.5127-38
Source: PubMed

ABSTRACT Migration and proliferation have been mostly explored in culture systems or fixed preparations. We present a simple genetic model, the chains of glia moving along fly wing nerves, to follow such dynamic processes by time-lapse in the whole animal. We show that glia undergo extensive cytoskeleton and mitotic apparatus rearrangements during division and migration. Single cell labelling identifies different glia: pioneers with high filopodial, exploratory, activity and, less active followers. In combination with time-lapse, altering this cellular environment by genetic means or cell ablation has allowed to us define the role of specific cell-cell interactions. First, neurone-glia interactions are not necessary for glia motility but do affect the direction of migration. Second, repulsive interactions between glia control the extent of movement. Finally, autonomous cues control proliferation.

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Keywords

autonomous cues control proliferation
 
cell ablation
 
cellular environment
 
culture systems
 
different glia
 
dynamic processes
 
exploratory
 
extensive cytoskeleton
 
glia
 
glia control
 
glia motility
 
mitotic apparatus rearrangements
 
neurone-glia interactions
 
repulsive interactions
 
simple genetic model
 
Single cell labelling
 
specific cell-cell interactions
 
whole animal
 
wing nerves