Sequence-based typing of flaB is a more stable screening tool than typing of flaA for monitoring of Campylobacter populations.

Alexander Mellmann, Jan Mosters, Edda Bartelt, Peter Roggentin, Andrea Ammon, Alexander W Friedrich, Helge Karch, Dag Harmsen

Institut für Hygiene, Universitätsklinikum Münster, Münster, Germany.

Journal Article: Journal of Clinical Microbiology (impact factor: 4.16). 11/2004; 42(10):4840-2. DOI: 10.1128/JCM.42.10.4840-4842.2004

Abstract

Different typing schemes for Campylobacter spp. were evaluated with 70 outbreak and sporadic isolates. The discriminatory indexes were 0.944 (by pulsed-field gel electrophoresis), 0.920 (by genotyping of the flagellin A gene), 0.902 (by genotyping of flaB), and 0.886 (by multilocus sequence typing). Cross-classification gave 94.77 or 95.82% (PFGE-flaA or PFGE-flaB) concordance. flaA was overdiscriminatory in three cases, most probably due to intragenomic recombination.

Source: PubMed

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Keywords

70 outbreak
 
Cross-classification
 
Different typing schemes
 
flaB
 
genotyping
 
intragenomic recombination
 
multilocus sequence typing
 
PFGE-flaB
 
pulsed-field gel electrophoresis