Article

Utilization of enzymes for environmental applications.

Human Genome Sciences, Inc., Rockville, MD, USA.
Critical Reviews in Biotechnology (Impact Factor: 7.84). 02/2004; 24(2-3):125-54. DOI: 10.1080/07388550490493726
Source: PubMed

ABSTRACT Enzymes are powerful tools that help sustain a clean environment in several ways. They are utilized for environmental purposes in a number of industries including agro-food, oil, animal feed, detergent, pulp and paper, textile, leather, petroleum, and specialty chemical and biochemical industry. Enzymes also help to maintain an unpolluted environment through their use in waste management. Recombinant DNA technology, protein engineering, and rational enzyme design are the emerging areas of research pertaining to environmental applications of enzymes. The future will also see the employment of various technologies including gene shuffling, high throughput screening, and nanotechnology. This article presents an overview of the enzymatic applications in pollution control and the promising research avenues in this area.

2 Bookmarks
 · 
259 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: The fresh waste activated sludge (WAS) from a lab-scale sequencing batch reactor was used to determine the performance of the lysozyme for promoting its biodegradability. The results showed that a strict linear relationship presented between the degree of disintegration (DDM) of WAS and the lysozyme incubation time from 0 to 240min (R(2) was 0.992, 0.995 and 0.999 in accordance with the corresponding lysozyme/TS, respectively). Ratio of net SCOD increase augmented significantly by lysozyme digestion for evaluating the sludge biodegradability changes. Moreover, the protein dominated both in the EPS and SMP. In addition, the logarithm of SMP contents in supernatant presented an increasing trend similar with the ascending logarithmic relation with the lysozyme incubation time from 0 to 240min (R(2) was 0.960, 0.959 and 0.947, respectively). The SMP, especially the soluble protein, had an important contribution to the improvement of WAS biodegradability.
    Bioresource Technology 08/2014; 170C:108-114. · 5.04 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The environmental management facet is the most important part to be considered by the engineers and technocrats along with the management professionals to build a technology which will guarantee a better world to reside as it requires technical competence and scientific excellence. The modern manufacturing production and processes thus must be extremely flexible, environmentally respectable, sustain any of the frequent market fluctuations with ―sustainability‖ both environmentally and economically. Solid State Fermentation has been used for the production of enzymes, organic acids, food animal feed,and both pharmaceutical and agricultural product.
    International Journal of Emerging Trends in Engineering and Development. 01/2014; 41.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Three samples of soil were collected from the hot springs from Kotli Azad Jammu and Kashmir. Two samples were collected from the West and North Banks respectively and the third one was collected from inside the hot water of the spring. For serial dilutions 1g soil was dissolved in 100 ml of sterilized distilled water. The soil solution was further diluted up to thousand times. Out of these dilutions 10 ml sample was taken in test tube and kept at 90°C in an oven to give heat shock for duration of 15 minutes. The 1ml dilution was spread on the AGS medium. Out of 20 strains six giving higher zones of hydrolysis (N3, W1, W3 U1, U2 and U4) were inoculated nutrient starch agar slants. Four strains of bacteria N3, W1, U1 and U4 showing higher zones of hydrolysis i.e. 3.6, 4.2, 3.8 and 3.6 mm respectively. Strains N3, U1 and U2 were found to be bacillus and W1 was identified as Actinomycetes. On the basis of morphological and physiological tests as determined by Bergeys manual of bacteriology W-1 was identified as Thermoactinomyces sacchari. Identification was also done by PCR for which the forward and the reverse primers were prepared by the fermentos company USA. In PCR 2,3,6,8 lane gave positive results and band formation. Morphological identification and the biochemical tests confirmed that the strain was Thermoactinomyces sacchari so the strain was selected for further study. Different types of media were utilized for the maximum production. Growth of Actinomycete and amylase production was optimized on four different nutrient media. Highest growth and amylase activity (3.882 OD and 132.3 AU, respectively after 72 hours at 60°C) were obtained by using medium-4 and it is also called as AGS medium.
    World Applied Sciences Journal 02/2014; 30(3):350-354. · 0.23 Impact Factor

Full-text

Download
10 Downloads
Available from