Article

Substance P enhances expression of lipopolysaccharide-induced inflammatory factors in dental pulp cells.

Department of Operative Dentistry and Endodontology, Kagoshima University Dental School, Kagoshima, Japan.
Journal of Endodontics (impact factor: 2.88). 12/2004; 30(11):770-3. pp.770-3
Source: PubMed

ABSTRACT To examine how substance P (SP) is related with dental pulp inflammation, we examined the effects of SP on expression of genes for inflammatory factors in human dental pulp cell cultures. Using reverse transcriptase-polymerase chain reaction, we found that Prevotella intermedia lipopolysaccharide (LPS) induced expression of SP and SP-receptor mRNAs, and that somatostatin inhibited the LPS-induced expression of SP mRNA. We also found that SP enhanced LPS-induced stimulation of NF-kappaB binding activity. In addition, SP induced expression of cyclooxygenase-2 and interleukin-10 receptor mRNAs. In contrast, SP inhibited expression of interferon-gamma receptor mRNA. These results suggest that SP may play a regulatory role in the immunological response of dental pulp tissue to pathogenic bacteria.

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Keywords

dental pulp inflammation
 
dental pulp tissue
 
human dental pulp cell cultures
 
immunological response
 
inflammatory factors
 
interferon-gamma receptor mRNA
 
interleukin-10 receptor mRNAs
 
LPS
 
LPS-induced expression
 
LPS-induced stimulation
 
NF-kappaB binding activity
 
Prevotella intermedia lipopolysaccharide
 
regulatory role
 
reverse transcriptase-polymerase chain reaction
 
somatostatin inhibited
 
SP induced expression
 
SP inhibited expression
 
SP mRNA
 
SP-receptor mRNAs
 
substance P
 

Masayuki Tokuda