Estradiol and testosterone concentrations in follicular fluid as criteria to discriminate between mature and immature oocytes.

Setor de Reproducão Humana, Departamento de Ginecologia e Obstetrícia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.
Brazilian Journal of Medical and Biological Research (Impact Factor: 1.03). 12/2004; 37(11):1747-55. DOI: 10.1590/S0100-879X2004001100021
Source: PubMed

ABSTRACT The objective of the present study was to examine the association between follicular fluid (FF) steroid concentration and oocyte maturity and fertilization rates. Seventeen infertile patients were submitted to ovulation induction with urinary human follicle-stimulating hormone, human menopausal gonadotropin and human chorionic gonadotropin (hCG). A total of 107 follicles were aspirated after hCG administration, the oocytes were analyzed for maturity and 81 of them were incubated and inseminated in vitro. Progesterone, estradiol (E2), estrone, androstenedione, and testosterone were measured in the FF. E2 and testosterone levels were significantly higher in FF containing immature oocytes (median = 618.2 and 16 ng/ml, respectively) than in FF containing mature oocytes (median = 368 and 5.7 ng/ml, respectively; P < 0.05). Progesterone, androstenedione and estrone levels were not significantly different between mature and immature oocytes. The application of the receiver-operating characteristic curve statistical approach to determine the best cut-off point for the discrimination between mature and immature oocytes indicated levels of 505.8 ng/ml for E2 (81.0% sensitivity and 81.8% specificity) and of 10.4 ng/ml for testosterone (90.9% sensitivity and 82.4% specificity). Follicular diameter was associated negatively with E2 and testosterone levels in FF. There was a significant increase in progesterone/testosterone, progesterone/E2 and E2/testosterone ratios in FF containing mature oocytes, suggesting a reduction in conversion of C21 to C19, but not in aromatase activity. The overall fertility rate was 61% but there was no correlation between the steroid levels or their ratios and the fertilization rates. E2 and testosterone levels in FF may be used as a predictive parameter of oocyte maturity, but not for the in vitro fertilization rate.


Available from: Marcos Felipe Silva de Sá, Oct 24, 2014
1 Follower
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Résumé. Depuis le développement des techniques d'aide médicale à la procréation, des investigations pour élucider la contribution potentielle des désordres de la génomique, de la transcriptomique et de la protéomique dans les échecs des tentatives de FIV ont été rappor-tées dans la littérature. Les études ont été menées grâce à diverses approches techniques, telles que l'étude de l'expression des gènes et de l'ensemble des protéines dans les cellules de la granulosa mais aussi dans l'ovocyte lui-même, essentiellement chez l'animal. Bien que toutes ces études n'aient pas encore permis d'établir un profil moléculaire permettant de prédire la qualité d'un ovocyte, elles ont généré une quantité d'informations intéressantes concer-nant le protéome du liquide folliculaire et de l'ovocyte. Pour améliorer la prise en charge des couples infertiles, l'identification de marqueurs moléculaires permettant de prédire la qualité de l'ovocyte, de l'embryon ou de l'endomètre deviendra probablement utile et nécessaire dans nos pratiques d'investigations et de thérapeutiques dans le proche futur. Mots clés : liquide folliculaire, ovocyte, protéomique
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The aim of this study was to trigger the expression of genes related to oocytes in putative ovarian stem cells scraped from the ovarian surface epithelium of women with premature ovarian failure and cultured in vitro in the presence of follicular fluid, rich in substances for oocyte growth and maturation. Ovarian surface epithelium was scraped and cell cultures were set up by scrapings in five women with nonfunctional ovaries and with no naturally present mature follicles or oocytes. In the presence of donated follicular fluid putative stem cells grew and developed into primitive oocyte-like cells. A detailed single-cell gene expression profiling was performed to elucidate their genetic status in comparison to human embryonic stem cells, oocytes, and somatic fibroblasts. The ovarian cell cultures depleted/converted reproductive hormones from the culture medium. Estradiol alone or together with other substances may be involved in development of these primitive oocyte-like cells. The majority of primitive oocyte-like cells was mononuclear and expressed several genes related to pluripotency and oocytes, including genes related to meiosis, although they did not express some important oocyte-specific genes. Our work reveals the presence of putative stem cells in the ovarian surface epithelium of women with premature ovarian failure.
    02/2013; 2013:861460. DOI:10.1155/2013/861460
  • [Show abstract] [Hide abstract]
    ABSTRACT: Steroidogenic tissues such as the ovary, testes or adrenal glands are paradoxical in that they often indicate actions of steroid hormones within a dynamic range of ligand concentration in a high nanomolar or even micromolar level, i.e. at the natural concentrations existing within those organs. Yet ligand-activated nuclear steroid receptors act classically by direct interaction with DNA in the picomolar or low nanomolar range. Moreover, global genomic studies suggest that less than 40% of steroid-regulated genes involve classical responsive elements in gene promoter regions. The bovine oxytocin gene is a key element in the maternal recognition of pregnancy in ruminants and is regulated via an SF1 site in its proximal promoter. This gene is also regulated by steroids acting in a non-classical manner, involving nuclear receptors which do not interact directly with DNA. Dose-response relationships for these actions are in the high nanomolar range. Similar 'steroid sensing' mechanisms may prevail for other SF1-regulated genes and predict alternative pathways by which environmental endocrine disruptors might influence the functioning of steroid-producing organs and hence indirectly the steroid-dependent control of physiology and development.
    Molecular and Cellular Endocrinology 04/2013; 382(1). DOI:10.1016/j.mce.2013.04.016 · 4.24 Impact Factor