Short report: fluorescent Leishmania: application to anti-leishmanial drug testing.

Neeloo Singh, Anuradha Dube

Division of Biochemistry and Division of Parasitology, Central Drug Research Institute, Lucknow, India.

Journal Article: The American journal of tropical medicine and hygiene (impact factor: 2.8). 11/2004; 71(4):400-2.

Abstract

Classic techniques for detecting the susceptibility of Leishmania to different drugs are time-consuming, laborious, and require the use of macrophages. The use of flow cytometry for monitoring Leishmania susceptibility to drugs is beginning to be implemented. Using green fluorescent protein (GFP), we have improved and simplified the screening procedure. We introduced a GFP marker into field strains of Leishmania causing kala-azar (visceral leishmaniasis) and explored the suitability of transgenic L. donovani promastigotes that constitutively express GFP in their cytoplasm as target cells for in vitro screening of anti-leishmanial drugs.

Source: PubMed

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Keywords

Classic techniques
 
cytoplasm
 
different drugs
 
field strains
 
flow cytometry
 
GFP
 
GFP marker
 
green fluorescent protein
 
laborious
 
monitoring Leishmania susceptibility
 
susceptibility
 
target cells
 
transgenic L. donovani promastigotes
 
visceral leishmaniasis