Article

Heterodimerization and endocytosis of Arabidopsis brassinosteroid receptors BRI1 and AtSERK3 (BAK1).

Laboratory of Biochemistry, Wageningen University, 6703 HA Wageningen, The Netherlands.
The Plant Cell (impact factor: 8.99). 01/2005; 16(12):3216-29. DOI:10.1105/tpc.104.025387 pp.3216-29
Source: PubMed

ABSTRACT In Arabidopsis thaliana brassinosteroid (BR), perception is mediated by two Leu-rich repeat receptor-like kinases, BRASSINOSTEROID INSENSITIVE1 (BRI1) and BRI1-ASSOCIATED RECEPTOR KINASE1 (BAK1) (Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR-like KINASE3 [AtSERK3]). Genetic, biochemical, and yeast (Saccharomyces cerevisiae) interaction studies suggested that the BRI1-BAK1 receptor complex initiates BR signaling, but the role of the BAK1 receptor is still not clear. Using transient expression in protoplasts of BRI1 and AtSERK3 fused to cyan and yellow fluorescent green fluorescent protein variants allowed us to localize each receptor independently in vivo. We show that BRI1, but not AtSERK3, homodimerizes in the plasma membrane, whereas BRI1 and AtSERK3 preferentially heterodimerize in the endosomes. Coexpression of BRI1 and AtSERK3 results in a change of the steady state distribution of both receptors because of accelerated endocytosis. Endocytic vesicles contain either BRI1 or AtSERK3 alone or both. We propose that the AtSERK3 protein is involved in changing the equilibrium between plasma membrane-located BRI1 homodimers and endocytosed BRI1-AtSERK3 heterodimers.

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Keywords

Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR-like KINASE3 [AtSERK3]
 
Arabidopsis thaliana brassinosteroid
 
AtSERK3 fused
 
AtSERK3 preferentially heterodimerize
 
AtSERK3 protein
 
AtSERK3 results
 
BAK1 receptor
 
BRASSINOSTEROID INSENSITIVE1
 
BRI1-ASSOCIATED RECEPTOR KINASE1
 
BRI1-BAK1 receptor complex initiates BR signaling
 
Coexpression
 
endocytosed BRI1-AtSERK3 heterodimers
 
Leu-rich repeat receptor-like kinases
 
plasma membrane
 
plasma membrane-located BRI1 homodimers
 
receptor
 
receptors
 
Saccharomyces cerevisiae
 
transient expression
 
yellow fluorescent green fluorescent protein variants