Human skin cell cultures onto PLA50 (PDLLA) bioresorbable polymers: influence of chemical and morphological surface modifications.
ABSTRACT Poly(alpha-hydroxy acid)s derived from lactic and glycolic acid are bioresorbable polymers which can cover a large range of thermal, physical, mechanical, and biological properties. Human keratinocytes have been shown as able to grow on a poly(DL-lactic acid) film. However the keratinocyte growth was delayed with respect to culture on standard tissue culture polystyrene, even though the same plateau level was observed after 2 weeks. In order to improve the performance of poly(DL-lactic acid) films as skin culture support, their surface was modified by creating tiny cavities using a method based on the leaching out of poly(ethylene oxide) from poly(lactic acid)-poly(ethylene oxide) heterogeneous blends. The surface of the films was also chemically modified by alkaline attack with sodium hydroxide and by type-I collagen coating. Murine fibroblast cell line and primary cultures of human fibroblasts and of two types of keratinocytes were allowed to adhere and to grow comparatively on the different films. The presence of cavities affected neither the adhesion of dermal fibroblasts nor that of keratinocytes. Only keratinocyte proliferation was significantly reduced by the presence of cavities. Collagen coating improved skin cell adhesion and proliferation as well, except in the case of murine fibroblasts. In the case of the NaOH treatments, similar trends were observed but their extent depended on the treatment time. In the case of chemical modifications, fluorescence microscopy bore out adhesion and proliferation tendencies deduced from MTT tests.
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ABSTRACT: The purpose of this work was an optimization of polylactide film surfaces designed for human keratinocytes cultivation. The polylactide films were coated by collagen 1. The experiments showed that uniform covering of polymer surface by collagen, and formation of different collagen structures depend on the mode of the protein application. The differences in collagen distribution on the polymer surface influened the keratinocytes growth in culture. Analysis of keratinocytes alignment, as well as cytoskeleton organization demonstrated that fibrillar collagen promoted more even keratinocytes distribution in comparison with the distribution on molecular collagen.Tsitologiia 02/2007; 49(1):32-9.
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ABSTRACT: Biodegradable polymers have received increasing interests for biomedical applications. The surface morphology of biodegradable polymer films plays an important role for their applications in cell growth and tissue engineering. The conventional processes for the preparation of biodegradable polymer films are mainly based on solution-casting and melting methods, which are time-consuming and tend to be uncontrollable. For a better control of the surface morphology, an aerosol-assisted deposition process has been developed for the deposition of biodegradable poly (d,l-lactic acid) (PDLLA) films. It involves atomizing and transporting polymer fine droplets towards a heated substrate. With the evaporation of solvent, PDLLA films can be deposited rapidly on the substrate. In the aerosol-assisted deposition process, the thickness of the films can be controlled by the deposition time, and the surface morphology of the films is adjustable from highly porous to dense structure.Surface and Coatings Technology 07/2008; 202(21):5175-5179. DOI:10.1016/j.surfcoat.2008.05.055 · 2.20 Impact Factor