Prior PCB exposure suppresses hypoxia-induced up-regulation of glycolytic enzymes in Fundulus heteroclitus. Comp Biochem Physiol

University of British Columbia - Vancouver, Vancouver, British Columbia, Canada
Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology (Impact Factor: 2.3). 11/2004; 139(1-3):23-9. DOI: 10.1016/j.cca.2004.08.015
Source: PubMed


Increased activity of the glycolytic enzymes is a conserved feature of the cellular response to hypoxia, and may represent a protective mechanism by which cells can survive short-term hypoxic exposure. Gene induction by hypoxia involves a dimer of the hypoxia inducible factor (HIF)-1 alpha and the nuclear cofactor HIF-1 beta, also called the aryl hydrocarbon receptor nuclear translocator (ARNT), which is also involved in induction of genes in response to aryl hydrocarbon exposure. To assess the possibility of interaction between these pathways, we examined changes in the activity of the glycolytic enzymes in response to hypoxia and polychlorinated biphenyl (PCB) exposure in the liver of a teleost fish, Fundulus heteroclitus. After 3 days of hypoxic exposure (dissolved oxygen levels between 1.5 and 2.0 mg/L), there were significant increases in the activity of six glycolytic enzymes (PGI, ALD, TPI, PGK, PGM and LDH). In contrast, intraperitoneal injection of 1 microg/g body weight of PCB #77 (3,3',4,4'-tetrachlorobiphenyl) caused significant decreases in glycolytic enzyme activity after 7 days of exposure. When fish were injected with PCB #77 and then (4 days later) exposed to hypoxia for 3 days as before, we observed no induction of the glycolytic enzymes. This suggests that there is an antagonistic interaction between exposure to PCBs and hypoxia in F. heteroclitus. Prior PCB exposure could make these fish less tolerant of environmental hypoxia.

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    • "Few studies have assessed the effects of PCB exposure on the hypoxia signaling pathway. A study by Kraemer and Schulte assessed the effect of PCB 77, another dioxin-like PCB, on hypoxia-induced glycolytic enzyme activity in fish and found that PCB treatment decreased enzyme activity (Kraemer and Schulte, 2004). However, it is not known if PCB 126 affects hypoxia-regulated responses of HIF-1␣. "
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    ABSTRACT: Aerobic organisms strongly depend on the availability of oxygen for respiration and countless other metabolic processes to maintain cellular homeostasis. Under certain conditions, the amount of available oxygen can be limited. To support survival in environments with limited oxygen supply, hypoxia-inducible factors (HIFs) reprogram vital components of cellular metabolism. HIF-1α is an important mediator of acute and adaptive responses to hypoxic stress. Interestingly, the heterodimeric partner required by HIF-1α to function as transcription factor, known as ARNT, is also an essential part of the aryl hydrocarbon receptor (AhR) transcription factor complex. Thus, via ARNT a crosstalk exists between these two pathways that might affect HIF-1α-mediated processes. In this study we sought to assess the effect of the AhR agonist PCB 126 on HIF-1α activity as well as on HIF-1α-regulated targets involved in cellular metabolism in human HepG2 cells. Our results show that PCB 126 reduced HIF-1α localization to the nucleus. Furthermore, in an in vivo setting, rats exposed to parenteral PCB 126 also displayed reduced hepatocyte nuclear localization of HIF-1α. Additionally, HepG2 cells exposed to PCB 126 displayed reduced hypoxia-regulated HRE-luciferase reporter gene expression as well as a reduction in glucose consumption in conditions of hypoxia. In summary, this study reveals that HIF-1α-regulated cellular metabolic processes are negatively affected by PCB 126 which might ultimately affect adaptive responses and cell survival in hypoxic environments.
    Experimental and toxicologic pathology: official journal of the Gesellschaft fur Toxikologische Pathologie 06/2014; 66(8). DOI:10.1016/j.etp.2014.05.005 · 1.86 Impact Factor
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    • "In this regard, it has been discussed by others that hypoxia promote a transient increase in blood glucose due to a delayed utilization rate during the switch on substrate fuel preferences , namely from protein and lipids to carbohydrates (Haman et al., 1997; Vianen et al., 2002; Speers-R oesch et al., 2010 ). It is interesting to mention that a previous study by Kraemer and Schulte (2004), detected that PCBs pre-treatm ent inhibits the hypoxia-me diated induction on some glycolyti c enzymes of Fundulus heteroclitus . Conceivably, BaP-treatment may be interfere d with the hypoxia-mediated change in energy substrates to fuel glycolysis, resulting in the transient glucose increase detected in our study. "
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    ABSTRACT: Aquatic hypoxia is a seasonal condition in some coastal and continental wetlands where co-exposure with polycyclic aromatic hydrocarbons (PAHs) pollution may be detrimental to the biota. In the present study, adult tilapia, an euryoxic fish of high economic importance, were intraperitoneally injected with benzo[a]pyrene (BaP) (20mgkg(-1)) and then exposed to graded hypoxia to assess combined effects on some detoxification and fitness parameters. Seventy-two hours after a stepped decrease in dissolved oxygen (<2mgL(-1)), BaP treatment resulted in a significant diminution on the biliary BaP concentration (70% of normoxic group) and an increase in blood glucose levels (2.17-fold compared with normoxic group). These effects returned to control values in the following 48h of hypoxia exposure. BaP-induced CYP1A mRNA levels were unaffected by hypoxia, suggesting that reduced bile BaP concentration may be related with effects on protein amount or enzyme activities. LDH mRNA levels, blood lactate and hematocrit remained without change, suggesting no extreme detrimental effects for tilapia in the short-term of the BaP-hypoxia challenge. Our results indicate that BaP treatment and hypoxia targeted glucose metabolism and biliary BaP elimination, probably by favoring the storage of BaP in tilapia tissues.
    Chemosphere 04/2013; 92(1). DOI:10.1016/j.chemosphere.2013.03.034 · 3.34 Impact Factor
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    • "Studies have also shown that hypoxia may increase the toxicity of exposures to PAHs, dioxins, and PCBs, leading to potential CITS 102, 103. Exposures to these chemical classes may also hinder the ability of species to respond to increased hypoxia under climate change (TICS scenario) 104. Interactions between hypoxia and contaminants demonstrate the complexity of direct and indirect parameters altered by GCC that could impair the health of aquatic organisms and populations. "
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    ABSTRACT: Incorporation of global climate change (GCC) effects into assessments of chemical risk and injury requires integrated examinations of chemical and nonchemical stressors. Environmental variables altered by GCC (temperature, precipitation, salinity, pH) can influence the toxicokinetics of chemical absorption, distribution, metabolism, and excretion as well as toxicodynamic interactions between chemicals and target molecules. In addition, GCC challenges processes critical for coping with the external environment (water balance, thermoregulation, nutrition, and the immune, endocrine, and neurological systems), leaving organisms sensitive to even slight perturbations by chemicals when pushed to the limits of their physiological tolerance range. In simplest terms, GCC can make organisms more sensitive to chemical stressors, while alternatively, exposure to chemicals can make organisms more sensitive to GCC stressors. One challenge is to identify potential interactions between nonchemical and chemical stressors affecting key physiological processes in an organism. We employed adverse outcome pathways, constructs depicting linkages between mechanism-based molecular initiating events and impacts on individuals or populations, to assess how chemical-and climate-specific variables interact to lead to adverse outcomes. Case examples are presented for prospective scenarios, hypothesizing potential chemical-GCC interactions, and retrospective scenarios, proposing mechanisms for demonstrated chemical-climate interactions in natural populations. Understanding GCC interactions along adverse outcome pathways facilitates extrapolation between species or other levels of organization, development of hypotheses and focal areas for further research, and improved inputs for risk and resource injury assessments. Environ. Toxicol. Chem. © 2012 SETAC.
    Environmental Toxicology and Chemistry 01/2013; 32(1). DOI:10.1002/etc.2043 · 3.23 Impact Factor
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