Cytotoxicity Evaluation of Soft Contact Lens Care Solutions on Human Conjunctival Fibroblasts
Department of Ophthalmology, Medical University Graz, Graz, Austria. Ophthalmologica
(Impact Factor: 1.68).
11/2004; 218(6):385-9. DOI: 10.1159/000080941
To determine whether different contact lens care solutions for soft lenses cause damage to human conjunctival cells.
Primary cultured human conjunctival fibroblasts were incubated with various concentrations of four different commercially available soft contact lens care solutions (OptiFree, Renu, SoloCare, Titmus) at concentrations of 5, 10 and 50 microl/ml medium. Toxicity was examined by determination of (1) the cell viability and mitochondrial activity with the colorimetric MTT test, and (2) the number of living cells with a cell analysis system (CASY 1) as compared with untreated cells.
For all four soft contact lens care solutions at a concentration of 5 mul/ml medium, no significant decrease in mitochondrial activity of the human conjunctival fibroblasts was found by the MTT test. At 10 microl/ml, only OptiFree and Titmus reduced mitochondrial viability significantly. The greatest reduction in mitochondrial activity occurred with all of the four soft contact lens care solutions at a concentration of 50 microl/ml. No significant decrease in the number of living conjunctival fibroblasts was observed by CASY 1 even at higher concentrations of the four solutions investigated.
This in vitro study demonstrates that the examined soft contact lens care solutions induce changes in mitochondria of human conjunctival cells only at higher doses as observed by the MTT test. However, this damage to the mitochondria did not lead to cell death as shown by the cell analysis system.
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ABSTRACT: Rapid development of science and technology has given rise to a great quantity of high-tech enterprise, and science and technology in enterprises development have become increasingly important. The activities about science research and technology development have become an important content of study in the field of accounting. This study is certainly of great significance in the promotion of the technology innovation and continuous development of enterprise in the 21st Century. This paper discusses the accounting of research and development, which is thought to be a new branch of accounting and whose research goal is the accounting issues related to technology research and development of an enterprise, as well as providing accounting information reflecting the development potentiality of an enterprise
Management of Innovation and Technology, 2000. ICMIT 2000. Proceedings of the 2000 IEEE International Conference on; 02/2000
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ABSTRACT: Introduction: Chemical burns of the eye by acid or alkali may also damage conjunctival cells. We describe a method to measure the mitochondrial activity of human conjunctival cells. By this method the degree of injury to the cells by chemical agents can be determined. Method: Human conjunctival cells (Chang cells) in cell culture were incubated with hydrochloric acid (0.02 M; 0.05 M; 0.1 M HCl) or sodium hydroxide solution (0.02 M; 0.05 M; 0.1 M NaOH) for a defined period of time (10-60 sec). To determine the mitochondrial activity of the cells the Cell Titer 96® Aqueous Solution Cell Proliferation Assay was used. Results: Within few seconds the mitochondrial activity of the conjunctival cells decreased depending on the concentration of the acidic or alkaline solution and on the time of incubation. Conclusion: By this method the damage of human conjunctival cells by acid or alkali can be determined quantitatively. Evaluation of the activity of substances for cell regeneration after chemical burns is also possible.
Spektrum der Augenheilkunde 01/2006; 20(4):188-190. · 0.18 Impact Factor
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