Article

Lactobacillus plantarum inhibits the intestinal epithelial migration of neutrophils induced by enteropathogenic Escherichia coli.

Department of Pediatrics, Division of Gastroenterology and Nutrition, Wright State University and The Children's Medical Center, Dayton, Ohio 45404, USA.
Journal of Pediatric Gastroenterology and Nutrition (Impact Factor: 2.2). 04/2003; 36(3):385-91. DOI: 10.1097/00005176-200303000-00017
Source: PubMed

ABSTRACT Lactobacillus plantarum is a Gram-positive bacillus known for its effect as a probiotic agent. The goal of the study was to determine whether L. plantarum is capable of inhibiting the transepithelial neutrophil migration induced by enteropathogenic Escherichia coli (EPEC).
Cultured intestinal epithelial T-84 cell monolayers were rapidly infected with EPEC. L. plantarum or culture supernatants were added to the monolayers before and after the infection. Indium-labeled neutrophils were added to the basolateral side of inverted monolayers. After 150-minute incubation, radioactivity of the neutrophils that migrated in the physiologic direction was assayed, and the number of migrating neutrophils was calculated. L. plantarum was also added to the monolayers before and after EPEC infection, and the number of adherent EPEC was determined by plate counting.
EPEC-induced neutrophil migration and EPEC binding to monolayers were inhibited by viable L. plantarum but only when added to the monolayers before EPEC. Culture supernatants failed to inhibit the neutrophil migration.
These results suggest that L. plantarum is beneficial in inhibiting neutrophil migration induced by EPEC, but only when preincubated with host epithelia. Rather than an indirect effect through a secreted substance produced by the probiotic agent, its effect is direct and requires the presence of the bacterium.

0 Bookmarks
 · 
64 Views
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: It is found that the probiotic strains of Escherichia coli, G35 N 59 isolated from “Okarin” and from “ASAP” drug formulas, and the commensal strain 5–1 isolated from healthy human intestine, manifest higher specific growth rates, lower acidifying capacity during glucose fermentation in the external medium, and lower rates of decrease in redox potential in comparison with the wild-type strain (MC4100). At the same time, despite similar values of their membrane potentials, these bacteria differ essentially in total and N,N′-dicyclohexylcarbodiimide-sensitive rates of energy-dependent transmembrane H+ and K+ transport and display a low level of H2 production. It is suggested that the difference between their membrane characteristics reflects changes in the activity of proton-translocating F0F1-ATPase and is crucial for bacterial growth and probiotic activity of E. coli.
    Biochemistry (Moscow) Supplement Series A Membrane and Cell Biology 01/2007; 1(4):331-335.
  • Source
  • [Show abstract] [Hide abstract]
    ABSTRACT: Selective microbes used as probiotics can enhance epithelial cell protection. We have previously shown that a Lactobacillus plantarum strain 299v (Lp299v) has the ability to induce mucin genes. In the current study, we utilized a cytokine model of inflammation in cell culture to study the modulation of apoptosis by this probiotic. HT-29 cells were pre-incubated with the Lp299v or L. plantarum strain adh- (Lpadh-), a non-adherent derivative of Lp299v. Cells were challenged with a mixture of cytokines (TNF-α, IFN-γ, and IL-1a) to imitate conditions of inflammation. To assess for cell death, we evaluated TUNEL, multi-caspase, and caspase-3 and caspase-7 activity assays. There was a marked decrease in apoptosis as measured by TUNEL+ cells in samples pre-treated with Lp299v (18.7 ± 4.1%, p p 0.05). Similarly, caspase-3, caspase-7 activity was also reduced by Lp299v. Selected probiotics may confer an exogenous protective effect at the mucosal–luminal interface for intestinal epithelial cells via alteration of caspase-dependent apoptotic pathways.
    Probiotics and Antimicrobial Proteins 01/2011; 3(1):21-26.