A neurogenomics approach to gene expression analysis in the developing brain

Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN 38105, United States.
Molecular Brain Research (Impact Factor: 2). 01/2005; 132(2):116-27. DOI: 10.1016/j.molbrainres.2004.10.002
Source: PubMed


Secreted and transmembrane proteins provide critical functions in the signaling networks essential for neurogenesis. We used a genetic signal sequence gene trap approach to isolate 189 genes expressed during development in e16.5 whole head, e16.5 hippocampus and e14.5 cerebellum. Gene ontology programs were used to classify the genes into respective biological processes. Four major classes of biological processes known to be important during development were identified: cell communication, cell physiology processes, metabolism and morphogenesis. We used in situ hybridization to determine the temporal and spatial patterns of gene expression in the developing brain using this set of probes. The results demonstrate that gene expression patterns can highlight potential gene functions in specific brain regions. We propose that combining bioinformatics with the gene expression pattern is an effective strategy to identify genes that may play critical roles during brain development.

Download full-text


Available from: John Mccoy, Oct 08, 2015
28 Reads
  • Source
    • "In this regard, it is particularly useful to compare the embryonic expression patterns with those of the adult as some genes may exhibit distinct functions in the developing and adult brain. BGEM BGEM is a growing atlas of genes expressed in the developing and adult mouse brain (Jensen et al. 2004). BGEM is part of the GENSAT project (http://www. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Brain gene expression databases are providing an increasing amount of information to the neuroscience community. Most databases are focused on the adult mouse rather than embryonic development. Here we survey the major mouse gene expression databases for the developing brain. The high throughput in situ hybridization approach generates large volumes of gene expression data that can be compiled and examined in a relatively short period of time. It is of increasing importance to compare gene expression patterns of neurodevelopment in the brain in relation to the adult. Often clues to adult gene expression and gene function can be determined by examining embryonic development. It is our hope that once all genes are mapped in the brain from the embryo to the adult, studies can be conducting based on information derived from such databases in conjunction with other bioinformatics sources.
    The Journal of Physiology 10/2006; 575(Pt 2):343-6. DOI:10.1113/jphysiol.2006.112607 · 5.04 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Presently, science is moving from genomics to proteomics in order to get insight into the functional network of gene expression. Actually however, proteomics is much older than genomics and dates back to the introduction of the two-dimensional gel electrophoresis technique (2-DE) independently by Klose and O'Farrell. Based on this approach almost all cellular proteins can be separated. New developments in mass spectrometry allowed identification of single spots in the 2-DE protein pattern, including the underlying genes. Joachim Klose has focused his pioneering 2-DE studies on mouse models with special emphasis on quantitative protein variants. According to him, proteins are living molecules exhibiting a characteristic protein phenotype.
    Electrophoresis 08/2001; 22(14):2835-7. DOI:10.1002/1522-2683(200108)22:14<2835::AID-ELPS2835>3.0.CO;2-3 · 3.03 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Mammalian cells have developed complex mechanisms to identify DNA damage and activate the required response to maintain genome integrity. Those mechanisms include DNA damage detection, DNA repair, cell cycle arrest and apoptosis which operate together to protect the conceptus from DNA damage originating either in parental gametes or in the embryo's somatic cells. DNA repair in the newly fertilized preimplantation embryo is believed to rely entirely on the oocyte's machinery (mRNAs and proteins deposited and stored prior to ovulation). DNA repair genes have been shown to be expressed in the early stages of mammalian development. The survival of the embryo necessitates that the oocyte be sufficiently equipped with maternal stored products and that embryonic gene expression commences at the correct time. A Medline based literature search was performed using the keywords 'DNA repair' and 'embryo development' or 'gametogenesis' (publication dates between 1995 and 2006). Mammalian studies which investigated gene expression were selected. Further articles were acquired from the citations in the articles obtained from the preliminary Medline search. This paper reviews mammalian DNA repair from gametogenesis to preimplantation embryos to late gestational stages.
    Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 01/2007; 635(1):53-77. DOI:10.1016/j.mrrev.2006.09.002 · 3.68 Impact Factor
Show more