Preparative isolation and purification from Psoralea corylifolia by high speed counter current chromatography

College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, China.
Journal of Chromatography A (Impact Factor: 4.17). 12/2004; 1057(1-2):225-8. DOI: 10.1016/j.chroma.2004.09.049
Source: PubMed


Psoralen and isopsoralen were separated from Psoralea corylifolia by high-speed counter-current chromatography (HSCCC). A two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (5:5:4.5:5.5, v/v) was used for HSCCC separation, and yielded, from 100 mg of crude extract, 39.6 mg of psoralen and 50.8 mg of isopsoralen each at over 99% purity as determined by high performance liquid chromatography (HPLC). The identification of psoralen and isopsoralen were performed with 1H NMR and 13C NMR.

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    • "In the Ayurvedic Pharmacopoeia of India, several reports revealed the presence of essential oil, coumarins, alkaloids, flavonoids and terpenoids like beneficiary compounds in the seeds of P. corylifolia. The literature also reveals therapeutic action of P. corylifolia against various diseases such as asthma, diarrhea, alopecia aretae,[2] impotence, menstrual disorder, uterine hemorrhage, as well as showing antitumor,[3] antiallergic,[4] antioxidant,[5] insecticidal and antimicrobial activities. P. corlifolia seed extracts have been reported to stimulate immune system in mice. "
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    ABSTRACT: Psoralea corylifolia (Bakuchi), a weed, which possesses a highly potent and medicinally important compound psoralen. P. corylifolia has been widely exploited since ages for its biological potential. Fifteen root nodulating bacteria as pure culture collection (PCC) were isolated from P. corylifolia in India. Further, these strains were evaluated for their effect on the psoralen content in P. corylifolia. High performance liquid chromatography (HPLC) method was used for the estimation of psoralen in P. corylifolia seed extracts. The effectiveness of these rhizobial strains was assessed on the basis of screening of various plant growth promoting attributes. The 16S ribosomal RNA sequencing analysis revealed the identity of two most effective rhizobial isolates PCC2 and PCC7 as Rhizobium leguminosarum and Sinorhizobium meliloti, respectively. The R. leguminosarum PCC2 (JN546144) and Ensifer meliloti PCC7 (JN546145) strains showed solubilization of insoluble inorganic phosphate, secreted indole acetic acid (IAA), produced siderophore, showed ACC deaminase activity, and were positive for nodulation and nitrogen fixing genes. Seeds of P. corylifolia were bacterized with combination of R. leguminosarum PCC2 and Ensifer meliloti PCC7 along with their individual application that resulted in enhancement of various early vegetative and late reproduction parameters of plants in two consecutive field trials in the year 2009 and 2010. The psoralen content in the seeds of P. corylifolia was observed to be increased in the field trials where the combination of rhizobial strains PCC2 and PCC7 was used (2.79%) compared to control (1.91%). These findings indicate that rhizobial strains PCC2 and PCC7 showing good plant growth promoting attributes can be effective for increasing the psoralen content in the seeds of P. corylifolia to a certain level.
    Pharmacognosy Magazine 10/2013; 9(Suppl 1):S57-65. DOI:10.4103/0973-1296.117870 · 1.26 Impact Factor
    • "It is used in the treatment of various kinds of disorders, such as asthma, cough, nephritis, and others. The major components psoralen and isopsoralen have antitumor, antibacterial, and antiviral properties.[45] It is a good hair tonic and hence used in alopecia areata and hair loss.[2437] "
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    ABSTRACT: Plants have been the basis of many traditional medicines throughout the world for thousands of years and continue to provide new remedies to mankind. Plants have been one of the important sources of medicines since the beginning of human civilization. The recent resurgence of plant remedies resulted from several factors, such as effectiveness of plant medicines and lesser side effects compared with modern medicines. Psoralea corylifolia, commonly known as babchi, is a popular herb, which has since long been used in traditional Ayurvedic and Chinese medicine for its magical effects to cure various skin diseases. This plant is also pharmacologically studied for its chemoprotective, antioxidant, antimicrobial, and antiinflammatory properties. This review attempts to highlight the available literature on P. corylifolia with respect to its ethnobotany, pharmacognostic characteristics, traditional uses, chemical constituents, and summary of its various pharmacologic activities and clinical effects. Other aspects, such as toxicology and precautions are also discussed. This will be helpful to create interest toward babchi and may be useful in developing new formulations with more therapeutic and economical value.
    03/2010; 4(7):69-76. DOI:10.4103/0973-7847.65331
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    • "The seeds of Psoralea corylifolia L. (Fabaceae), well-known as traditional Chinese medicine " Buguzhi " , are widely used for the treatment of various kinds of disorders such as asthma, cough, nephritis, vitiligo and calvities [1] [2]. The constituents in P. corylifolia L. include coumarins and flavone components, such as psoralen, isopsoralen, psoralidin, neobavaisoflavone, bavachin, corylin, bavachalcone, and so forth [3]. "
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    ABSTRACT: Psoralea corylifolia L. (Fabaceae) is a widely used medical plant in China. This study was designed to screen and identify bioactive compounds with anticancer activity from the seeds of Psoralea corylifolia L. One volatile fraction (fraction I) and three other fractions (fraction II, III, IV) from methanol extraction of P. corylifolia L. were obtained. Bioactivities of these fractions were evaluated by the cytotoxicity on KB, KBv200, K562, K562/ADM cancer cells with MTT assay. Major components in the active fraction were identified by HPLC/MS(n). Fraction IV significantly inhibits the growth of cancer cells in a dose-dependent manner. The IC(50) values were 21.6, 24.4, 10.0 and 26.9, respectively. Psoralen and isopsoralen, isolated from fraction IV, were subject to bioactive assay and presented a dose-dependent anticancer activity in four cancer cell lines (KB, KBv200, K562 and K562/ADM). The IC(50) values of psoralen were 88.1, 86.6, 24.4 and 62.6, which of isopsoralen were 61.9, 49.4, 49.6 and 72.0, respectively. Apoptosis of tumor cell significantly increased after treated with psoralen and isopsoralen. Induction of apoptotic activity was confirmed by flow cytometry after staining with Annexin V/PI. These results suggested psoralen and isopsoralen contribute to anticancer effect of P. corylifolia L.
    Evidence-based Complementary and Alternative Medicine 02/2009; 2011(1741-427X):363052. DOI:10.1093/ecam/nen087 · 1.88 Impact Factor
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