The plant pathogen Erwinia amylovora produces acyl-homoserine lactone signal molecules in vitro and in planta.
ABSTRACT We report for the first time the production of acyl homoserine lactones (AHLs) by Erwina amylovora, an important quarantine bacterial pathogen that causes fire blight in plants. E. amylovora produces one N-acyl homoserine lactone [a N-(3-oxo-hexanoyl)-homoserine lactone or a N-(3-hydroxy-hexanoyl)-homoserine lactone] quorum sensing signal molecule both in vitro and in planta (pear plant). Given the involvement of AHLs in plant pathogenesis, we speculate that AHL-dependent quorum sensing could play an important role in the regulation of E. amylovora virulence.
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ABSTRACT: Erwinia piriflorinigrans is a necrotrophic pathogen of pear reported from Spain that destroys flowers but does not progress further into the host. We sequenced the complete genome of the type strain CFBP 5888(T) clarifying its phylogenetic position within the genus Erwinia, and indicating a position between its closest relative, the epiphyte Erwinia tasmaniensis and other plant pathogenic Erwinia spp. (i.e., the fire blight pathogen E. amylovora and the Asian pear pathogen E. pyrifoliae). Common features are the type III and type VI secretion systems, amylovoran biosynthesis and desferrioxamine production. The E. piriflorinigrans genome also provided the first evidence for production of the siderophore chrysobactin within the genus Erwinia sensu stricto, which up to now was mostly associated with phytopathogenic, soft-rot Dickeya and Pectobacterium species. Plasmid pEPIR37, reported in this strain, is closely related to small plasmids found in the fire blight pathogen E. amylovora and E. pyrifoliae. The genome of E. piriflorinigrans also gives detailed insights in evolutionary genomics of pathoadapted Erwinia.Systematic and Applied Microbiology 05/2013; · 3.29 Impact Factor
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ABSTRACT: This review describes the chemistry of the bacterial biofilms including the chemistry of their constituents and signalling compounds that mediate or inhibit the formation of biofilms. Systems are described with special emphasis, in which quorum sensing molecules (autoinducers) trigger the formation of biofilms. In the first instance, N-acyl-L-homoserine lactones (AHLs) are the focus of this review, whereas the inter-species signal known as furanosyl borate diester and peptide autoinducers used by Gram-positive bacteria are not discussed in detail. Since the first discovery of an AHL autoinducer from Vibrio fischeri a large and further increasing number of different AHL structures from Gram-negative bacteria have been identified. This review gives a summary of all known AHL autoinducers and producing bacterial species. A few systems are discussed, where biofilm formation is suppressed by enzymatic degradation of AHL molecules or interference of secondary metabolites from other species with the quorum sensing systems of communicating bacteria. Finally, the multi-channel quorum sensing system, the intracellular downstream processing of the signal, and the resulting response of whole populations including biofilm formation are discussed for the Vibrio genus that has been extensively investigated.Natural Product Reports 03/2010; 27(3):343-69. · 10.18 Impact Factor
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ABSTRACT: SdiA of E. coli and Salmonella is a LuxR homolog that detects N-acyl homoserine lactones (AHLs). Most LuxR homologs function together with a cognate AHL synthase (a LuxI homolog), but SdiA does not. Instead, SdiA detects AHLs produced by other bacterial species. In this report, we performed a phylogenetic analysis of SdiA. The results suggest that one branch of the Enterobacteriaceae obtained a rhlR/rhlI pair by horizontal transfer. The Erwinia and Pantoea branches still contain the complete pair where it is known as expR/expI and phzR/phzI, respectively. A deletion event removed the luxI homolog from the remainder of the group, leaving just the luxR homolog known as sdiA. Thus ExpR and PhzR are SdiA orthologs and ExpI and PhzI are descendants of the long lost cognate signal synthase of SdiA.PLoS ONE 01/2012; 7(10):e47720. · 3.53 Impact Factor