Article
The use of debrided human articular cartilage for autologous chondrocyte implantation: maintenance of chondrocyte differentiation and proliferation in type I collagen gels.
Department of Anatomy and Cell Biology, Monash University, Clayton, Vic 3800, Australia.
Journal of Orthopaedic Research (impact factor:
2.81).
04/2004;
22(2):446-55.
DOI:10.1016/j.orthres.2003.07.001
pp.446-55
Source: PubMed
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Citations (0)
- Cited In (4)
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Article: Cell Seeding Densities in Autologous Chondrocyte Implantation Techniques for Cartilage Repair
[show abstract] [hide abstract]
ABSTRACT: Cartilage repair techniques have been among the most intensively investigated treatments in orthopedics for the past decade, and several different treatment modalities are currently available. Despite the extensive research effort within this field, the generation of hyaline cartilage remains a considerable challenge. There are many parameters attendant to each of the cartilage repair techniques that can affect the amount and types of reparative tissue generated in the cartilage defect, and some of the most fundamental of these parameters have yet to be fully investigated. For procedures in which in vitro–cultured autologous chondrocytes are implanted under a periosteal or synthetic membrane cover, or seeded onto a porous membrane or scaffold, little is known about how the number of cells affects the clinical outcome. Few published clinical studies address the cell seeding density that was employed. The principal objective of this review is to provide an overview of the cell seeding densities used in cell-based treatments currently available in the clinic for cartilage repair. Select preclinical studies that have informed the use of specific cell seeding densities in the clinic are also discussed.Cartilage 01/2012; 3(2):108-117. -
Article: Clinical application of scaffolds for cartilage tissue engineering.
[show abstract] [hide abstract]
ABSTRACT: The purpose of this paper is to review the basic science and clinical literature on scaffolds clinically available for the treatment of articular cartilage injuries. The use of tissue-engineered grafts based on scaffolds seems to be as effective as conventional ACI clinically. However, there is limited evidence that scaffold techniques result in homogeneous distribution of cells. Similarly, few studies exist on the maintenance of the chondrocyte phenotype in scaffolds. Both of which would be potential advantages over the first generation ACI. The mean clinical score in all of the clinical literature on scaffold techniques significantly improved compared with preoperative values. More than 80% of patients had an excellent or good outcome. None of the short- or mid-term clinical and histological results of these tissue-engineering techniques with scaffolds were reported to be better than conventional ACI. However, some studies suggest that these methods may reduce surgical time, morbidity, and risks of periosteal hypertrophy and post-operative adhesions. Based on the available literature, we were not able to rank the scaffolds available for clinical use. Firm recommendations on which cartilage repair procedure is to be preferred is currently not known on the basis of these studies. Randomized clinical trials and longer follow-up periods are needed for more widespread information regarding the clinical effectiveness of scaffold-based, tissue-engineered cartilage repair.Knee Surgery Sports Traumatology Arthroscopy 12/2008; 17(6):561-77. · 2.21 Impact Factor -
Article: Cell Sources for Articular Cartilage Repair Strategies: Shifting from Monocultures to Cocultures.
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ABSTRACT: The repair of articular cartilage is challenging due to the sparse native cell population combined with the avascular and aneural nature of the tissue. In recent years, cartilage tissue engineering has shown great promise. As with all tissue engineering strategies, the possible therapeutic outcome is intimately linked with the used combination of cells, growth factors, and biomaterials. However, the optimal combination has remained a controversial topic and no consensus has been reached. In consequence, much effort has been dedicated, to further design, investigate, and optimize cartilage repair strategies. Specifically, various research groups have performed intensive investigations attempting to identify the single most optimal cell source for articular cartilage repair strategies. However, recent findings indicate that not the heavily investigated monocell source, but the less studied combinations of cell sources in coculture might be more attractive for cartilage repair strategies. This review will give a comprehensive overview on the cell sources that have been investigated for articular cartilage repair strategies. In particular, the advantages and disadvantages of investigated cell sources are comprehensively discussed with emphasis on the potential of cocultures in which benefits are combined, while the disadvantages of single-cell sources for cartilage repair are mitigated.Tissue Engineering Part B Reviews 07/2012; · 4.64 Impact Factor
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Keywords
'debrided human AC' cultured
'debrided' AC
4 weeks
4 weeks cultures
ACI
ACI procedure
cartilage specific matrix
cartilage-like matrix
cell proliferation
chondrocyte phenotype
debrided articular cartilage
healthy non-weight bearing area AC
large full thickness knee joint articular cartilage
normal articular cartilage
promising surgical treatment
routine surgical procedure
sufficient viable cell numbers
symptomatic knee full thickness AC defects
synthesized matrices
type II collagen
