Effects of fresh garlic extract on Candida albicans biofilms.
- SourceAvailable from: Ivan M. Savic[Show abstract] [Hide abstract]
ABSTRACT: Medical properties of garlic are mainly attributed to organosulfur compounds which are formed by enzymatic, chemical and thermal transformations of S-allyl-L-cysteine during crushing, drying or processing the bulb. Garlic has a bactericidal, bacteriostatic, antimicotic, antiviral, antisclerotic, antihypertensive, anti-aggregation and anticancer activity. The aim of this paper was to synthesize alliin from a genuine compound of deoxyalliin. Deoxyalliin is a main precursor for obtaining alliin which is contained in the garlic cloves. L-Cysteine and allyl bromide were used as the initial precursors for the synthesis of deoxyalliin. It is purified by recrystallization from absolute ethanol. The obtained deoxyalliin (>95 %) was used for the synthesis of alliin by oxidation with hydrogen peroxide. The structural characterization of synthesized deoxyalliin and alliin was studied by using UV, FTIR and MS spectrometry. The separation of optical alliin isomers was carried out by using a thin layer chromatography. The identification of synthetic compounds was achieved on the basis of literature data for Rf-values.
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ABSTRACT: Helicobacter pylori is a leading etiologic agent causing peptic ulcer and gastric cancer. The alternative lifestyle as a biofilm facilitates H. pylori to survive in adverse environments. Here, we investigated effect of curcumin on H. pylori biofilm formation both qualitatively by pellicle assay and quantitatively by crystal violet staining. Three-dimensional structure of biofilm was imaged by scanning electron microscopy. The effect of curcumin on H. pylori adherence to HEp-2 cells was also investigated. Sub-inhibitory concentrations of curcumin inhibited the biofilm in dose dependent manner. However, H. pylori could restore ability to form biofilm during extended time of incubation. Scanning electron microscopy revealed less amorphous extracellular polymeric matrix, slow of morphological conversion to coccoid form with cell damage after curcumin treatment. Curcumin significantly decreased the ability of H. pylori to adhere to the HEp-2 cells. Our findings demonstrated advantages of curcumin to inhibit biofilm formation by H. pylori, making it as a potential complimentary medicine for curing of H. pylori-biofilm related infections.AFRICAN JOURNAL OF BIOTECHNOLOGY 01/2002; 8:5106-5115. · 0.57 Impact Factor
- Kathmandu University Medical Journal 07/2007; 5(3):431-6.