Effects of fresh garlic extract on Candida albicans biofilms.

Antimicrobial Agents and Chemotherapy (Impact Factor: 4.57). 02/2005; 49(1):473. DOI: 10.1128/AAC.49.1.473.2005
Source: PubMed
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    ABSTRACT: When designing a human interface of a computer-mediated communication, it is important to take a socio-behavioral approach for understanding the nature of the communication. This study was conducted to investigate the users' social and behavioral characteristics in an online virtual world
    Robot and Human Communication, 1992. Proceedings., IEEE International Workshop on; 10/1992
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    ABSTRACT: Helicobacter pylori is a leading etiologic agent causing peptic ulcer and gastric cancer. The alternative lifestyle as a biofilm facilitates H. pylori to survive in adverse environments. Here, we investigated effect of curcumin on H. pylori biofilm formation both qualitatively by pellicle assay and quantitatively by crystal violet staining. Three-dimensional structure of biofilm was imaged by scanning electron microscopy. The effect of curcumin on H. pylori adherence to HEp-2 cells was also investigated. Sub-inhibitory concentrations of curcumin inhibited the biofilm in dose dependent manner. However, H. pylori could restore ability to form biofilm during extended time of incubation. Scanning electron microscopy revealed less amorphous extracellular polymeric matrix, slow of morphological conversion to coccoid form with cell damage after curcumin treatment. Curcumin significantly decreased the ability of H. pylori to adhere to the HEp-2 cells. Our findings demonstrated advantages of curcumin to inhibit biofilm formation by H. pylori, making it as a potential complimentary medicine for curing of H. pylori-biofilm related infections.
    AFRICAN JOURNAL OF BIOTECHNOLOGY 01/2002; 8:5106-5115. · 0.57 Impact Factor
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    ABSTRACT: Intercellular signal indole and its derivative hydroxyindoles inhibit Escherichia coli biofilm and diminish Pseudomonas aeruginosa virulence. However, indole and bacterial indole derivatives are unstable in the microbial community because they are quickly degraded by diverse bacterial oxygenases. Hence, this work sought to identify novel, non-toxic, stable and potent indole derivatives from plant sources for inhibiting the biofilm formation of E. coli O157:H7 and P. aeruginosa. Here, plant auxin 3-indolylacetonitrile (IAN) was found to inhibit the biofilm formation of both E. coli O157:H7 and P. aeruginosa without affecting its growth. IAN more effectively inhibited biofilms than indole for the two pathogenic bacteria. Additionally, IAN decreased the production of virulence factors including 2-heptyl-3-hydroxy-4(1H)-quinolone (PQS), pyocyanin and pyoverdine in P. aeruginosa. DNA microarray analysis indicated that IAN repressed genes involved in curli formation and glycerol metabolism, whereas IAN induced indole-related genes and prophage genes in E. coli O157:H7. It appeared that IAN inhibited the biofilm formation of E. coli by reducing curli formation and inducing indole production. Also, corroborating phenotypic results of P. aeruginosa, whole-transcriptomic data showed that IAN repressed virulence-related genes and motility-related genes, while IAN induced several small molecule transport genes. Furthermore, unlike bacterial indole derivatives, plant-originated IAN was stable in the presence of either E. coli or P. aeruginosa. Additionally, indole-3-carboxyaldehyde was another natural biofilm inhibitor for both E. coli and P. aeruginosa.
    Environmental Microbiology 01/2011; 13(1):62-73. · 6.24 Impact Factor


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