Proteomic analysis of the salt tolerance mutant of wheat under salt stress. Yi Chuan Xue Bao

College of Life Science, Hebei Normal University, Shijiazhuang 050016, China.
Acta Genetica Sinica 01/2005; 31(12):1408-14.
Source: PubMed


Two dimensional electrophoresis was used to analyse the proteome of the salt-tolerant mutant of wheat (RH8706-49) and the salt-sensitive mutant of wheat (H8706-34) which had been treated by 1% NaCl for 72 hours. After being analysed by MALDI-TOF-MS and Mascot software, the qualitative and quantitative differences were identified between the two materials for five candidate proteins: H+-transporting two-sector ATPase, glutamine synthetase 2 precursor, putative 33 kD oxygen evolving protein of photosystem II and ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit. These five proteins are all belong to chloroplast proteins. They are likely to play a crucial role in keeping the function of the chloroplast and the whole cells when the plant was under salt-stress.

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    • "In this study, number of Rubisco subunits was unevenly expressed in chloroplasts of water-stressed seedlings (Table 2). Isoforms of Rubisco activase likely play an important role in stabilizing and controlling proteolysis (Schwartz et al. 1995) and in maintaining chloroplast functioning during drought stress (Huo et al. 2004). Regulation of Rubisco activity has yet unclear under water stress. "
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    ABSTRACT: The performance of control and water-stressed 10-d-old wheat seedlings was compared. During short-term water stress (irrigation was withheld for 9 d), rates of photosynthesis and transpiration, stomatal conductance, and relative water content decreased whereas the proline content increased. Chloroplast proteins were extracted from the leaves, separated by iso-electric focusing through two-dimensional electrophoresis, and stained with CBB R-250. Differentially expressed proteins were detected and analyzed with MALDI-TOF/TOF mass spectrometry. Under water stress, 9 proteins were up-regulated whereas 11 proteins were not affected. The ribulose-1,5-bisphospate carboxylase/oxygenase (Rubisco) small and large subunits, chloride carrier/channel family, and H+-ATPase were up-regulated by water stress whereas membrane-bound ATP synthase subunit b and cytochrome b6-f complex were down-regulated.
    Biologia Plantarum 11/2012; 57(2). DOI:10.1007/s10535-012-0290-0 · 1.85 Impact Factor
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    • "The found drought stress up-regulates proteins in K86-161 cultivar such as protease inhibitor, RuBisCO activase isoform and ferredoxin are interesting. These proteins are likely to play a role in stabilizing, controlling proteolysis (Schwartz et al., 1995) and maintaining the function of the chloroplast during plant exposure to drought stress (Huo et al., 2004). When compared to control K86-161, the Protease inhibitor was only detected in stressed plant, thus this protein was induced by subsequent induction after stress treatment. "

    American Journal of Biochemistry and Biotechnology 04/2010; 6(2):89-102. DOI:10.3844/ajbbsp.2010.89.102
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    ABSTRACT: Drought is one of the most severe limitations on the productivity of rainfed lowland and upland rice. To investigate the initial response of rice to drought stress, changes in protein expression were analyzed using a proteomic approach. Two-week-old rice seedlings were exposed to drought conditions from 2 to 6 days, and proteins were extracted from leaf sheaths, separated by two-dimensional polyacrylamide gel electrophoresis and stained with Coomassie brilliant blue. After drought stress for 2 to 6 days, 10 proteins increased in abundance and the level of 2 proteins decreased. The functional categories of these proteins were identified as defense, energy, metabolism, cell structure, and signal transduction. In addition to drought stress, accumulations of protein were analyzed under several different stress conditions. The levels of an actin depolymerizing factor, a light harvesting complex chain II, a superoxidase dismutase and a salt-induced protein were changed by drought and osmotic stresses, but not cold or salt stresses, or abscisic acid treatment. The effect of drought stress on protein in the leaf sheaths of drought-tolerant rice cultivar was also analyzed. The light harvesting complex chain II and the actin depolymerizing factor were present at high levels in a drought-tolerant rice cultivar before stress application. With drought stress, actin depolymerizing factor was expressed in leaf blades, leaf sheaths, and roots. These results suggest that actin depolymerizing factor is one of the target proteins induced by drought stress.
    Journal of Proteome Research 03/2006; 5(2):396-403. DOI:10.1021/pr050291g · 4.25 Impact Factor
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