Proteomic analysis of the salt tolerance mutant of wheat under salt stress

College of Life Science, Hebei Normal University, Shijiazhuang 050016, China.
Acta Genetica Sinica 01/2005; 31(12):1408-14.
Source: PubMed

ABSTRACT Two dimensional electrophoresis was used to analyse the proteome of the salt-tolerant mutant of wheat (RH8706-49) and the salt-sensitive mutant of wheat (H8706-34) which had been treated by 1% NaCl for 72 hours. After being analysed by MALDI-TOF-MS and Mascot software, the qualitative and quantitative differences were identified between the two materials for five candidate proteins: H+-transporting two-sector ATPase, glutamine synthetase 2 precursor, putative 33 kD oxygen evolving protein of photosystem II and ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit. These five proteins are all belong to chloroplast proteins. They are likely to play a crucial role in keeping the function of the chloroplast and the whole cells when the plant was under salt-stress.

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    • "In this study, number of Rubisco subunits was unevenly expressed in chloroplasts of water-stressed seedlings (Table 2). Isoforms of Rubisco activase likely play an important role in stabilizing and controlling proteolysis (Schwartz et al. 1995) and in maintaining chloroplast functioning during drought stress (Huo et al. 2004). Regulation of Rubisco activity has yet unclear under water stress. "
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