Article

Proteomic analysis of the salt tolerance mutant of wheat under salt stress

College of Life Science, Hebei Normal University, Shijiazhuang 050016, China.
Acta Genetica Sinica 01/2005; 31(12):1408-14.
Source: PubMed

ABSTRACT Two dimensional electrophoresis was used to analyse the proteome of the salt-tolerant mutant of wheat (RH8706-49) and the salt-sensitive mutant of wheat (H8706-34) which had been treated by 1% NaCl for 72 hours. After being analysed by MALDI-TOF-MS and Mascot software, the qualitative and quantitative differences were identified between the two materials for five candidate proteins: H+-transporting two-sector ATPase, glutamine synthetase 2 precursor, putative 33 kD oxygen evolving protein of photosystem II and ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit. These five proteins are all belong to chloroplast proteins. They are likely to play a crucial role in keeping the function of the chloroplast and the whole cells when the plant was under salt-stress.

0 Followers
 · 
108 Views
  • Source
    • "In this study, number of Rubisco subunits was unevenly expressed in chloroplasts of water-stressed seedlings (Table 2). Isoforms of Rubisco activase likely play an important role in stabilizing and controlling proteolysis (Schwartz et al. 1995) and in maintaining chloroplast functioning during drought stress (Huo et al. 2004). Regulation of Rubisco activity has yet unclear under water stress. "
    [Show abstract] [Hide abstract]
    ABSTRACT: The performance of control and water-stressed 10-d-old wheat seedlings was compared. During short-term water stress (irrigation was withheld for 9 d), rates of photosynthesis and transpiration, stomatal conductance, and relative water content decreased whereas the proline content increased. Chloroplast proteins were extracted from the leaves, separated by iso-electric focusing through two-dimensional electrophoresis, and stained with CBB R-250. Differentially expressed proteins were detected and analyzed with MALDI-TOF/TOF mass spectrometry. Under water stress, 9 proteins were up-regulated whereas 11 proteins were not affected. The ribulose-1,5-bisphospate carboxylase/oxygenase (Rubisco) small and large subunits, chloride carrier/channel family, and H+-ATPase were up-regulated by water stress whereas membrane-bound ATP synthase subunit b and cytochrome b6-f complex were down-regulated.
    Biologia Plantarum 11/2012; DOI:10.1007/s10535-012-0290-0 · 1.74 Impact Factor
  • Source
  • [Show abstract] [Hide abstract]
    ABSTRACT: Soil salinity is a major abiotic constraint to agricultural productivity. We successfully bred a new common wheat (Triticum aestivum L.) introgression variety (Shanrong No. 3) with high salt-tolerance via asymmetric somatic hybridization between common wheat cultivar (Jinan 177) and UV-irradiated Agropyron elongatum (Thinopyrum ponticum Podp). We report here a comparative proteomic analysis to investigate variety-specific and salt-responsive proteins between seedling-roots of Shanrong No. 3 and Jinan 177. In total, 114 spots reproducibly presented differential expression patterns on 2-DE maps. Of them, 34 were variety-specific and 49 were salt-responsive. We identified 110 spots by MALDI-TOF MS and partially confirmed by MALDI-TOF-TOF MS, and functionally classified them into signal transduction, transcription and translation, transporting, chaperones, proteolysis and detoxification, etc. Meanwhile, we also found the alteration of protein expression of Shanrong No. 3 through inhibition of old proteins and production of novel ones, change in abundance and sensitivity of some nonsalt-responsive and salt-responsive proteins, as well as PTMs. Furthermore, comparison between proteome and transcripteome using cDNA microarray showed that there were only 20 proteins with abundances correlative to signal densities of corresponding EST probes. This study gives us a global insight into proteomic difference between Shanrong No. 3 and Jinan 177 in constitute and to salt-response.
    Proteomics 04/2008; 8(7):1470-89. DOI:10.1002/pmic.200700569 · 3.97 Impact Factor
Show more

Preview

Download
1 Download
Available from