Analysis of differentially expressed transcripts from planthopper-infested wild rice (Oryza minuta)

School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea.
Plant Cell Reports (Impact Factor: 3.07). 05/2005; 24(1):59-67. DOI: 10.1007/s00299-004-0905-9
Source: PubMed


A subtracted library was constructed from planthopper-infested wild rice (Oryza minuta) by suppression subtractive hybridization in combination with mirror orientation selection. To screen the differentially expressed transcripts in the library, we applied a cDNA microarray containing 960 random clones in a reverse Northern blot analysis using cDNA probes prepared from the mRNAs of control and planthopper-infested samples. On the basis of the signal intensities and expression ratios obtained from experiments performed in triplicate, we selected 383 clones. The elevated expression levels and overall profiles over time were verified by Northern blot analysis. Although Southern blot analysis showed similar copy numbers of the screened genes in O. minuta and O. sativa, it also revealed that the expression profiles had a different pattern. Functional categorization placed the identified transcripts in the categories of subcellular localization, metabolism, and protein fate. The presence of these expressed sequence tags implies that resistance of O. minuta to insect infestation can be achieved not only by an elevated expression of defense-related genes but also by enhanced metabolic activities.

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Available from: Ji-Ung Jeung, Jan 14, 2014
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    • "This discovery suggests that OsTPS13 is not a BPH feedinginducible STPS and that Os04g27430 expression is induced by both BPH feeding and other stresses, such as MeJA and wounding. Another STPS gene (Os08g07100) that is reportedly induced by BPH (Cho et al. 2005) was not polymorphic between KD and IL308 at the expression level in the present study (Figure 7A). This gene was induced by BPH feeding in both rice strains. "
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    ABSTRACT: Bph3, a major brown planthopper (BPH) resistance locus derived from the rice cultivar Rathu Heenati (RH), has been used as a stable donor of traits that improve highly susceptible aromatic rice varieties in Thailand. Map-based cloning was initiated using a set of isogenic lines (ILs) harboring the major Bph3 locus on chromosome 6. IL genomes were scanned with a 57 K Affymetrix Rice GeneChip to identify the gene responsible for Bph3. Single-feature polymorphism (SFP) mapping was used to localize 84 candidate genes. An expression analysis of 15 selected candidate genes in the aromatic rice cultivar KDML105 (KD) and the ILs under normal conditions revealed two differentially expressed sequences. Following hopper feeding, only one candidate gene, Os04g27430, was differentially expressed. Os04g27430 encodes a putative sesquiterpene synthase (STPS) gene that was induced by BPH feeding in ILs. An antixenosis test in three selected ILs revealed a major role for STPS in insect preference during the first 120 hours of the rice-insect interaction. Functional SNPs in exon 5 that resulted in the deletion of seven amino acids in the susceptible rice line were identified. Moreover, three additional SNPs associated with three transcription binding sites were also identified, which might explain the differential response of Os04g27430 during the anti-feeding test. Os04g27430 is the second known rice STPS induced by BPH. The gene may involve an antixenosis BPH resistance mechanism. The combination of the STPS and the Bph3 locus was more effective than Bph3 alone in the tested ILs.
    Rice 08/2013; 6(1):18. DOI:10.1186/1939-8433-6-18 · 3.92 Impact Factor
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    • "). Sap sacking insects cause a reduction in phenylpropanoid related transcripts. For example, in rice, brown planthopper feeding down-regulated several genes involved in phenylpropanoid biosynthesis and up-regulated a gene required for sesquiterpene synthesis (Cho et al. 2005 "
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    ABSTRACT: Background A major pest of rice, the Asian rice gall midge (Orseolia oryzae Wood-Mason), causes significant yield losses in the rice growing regions throughout Asia. Feeding by the larvae induces susceptible plants to produce nutritive tissue to support growth and development. In order to identify molecular signatures during compatible interactions, genome wide transcriptional profiling was performed using SSH library and microarray technology. Results Results revealed up-regulation of genes related to primary metabolism, nutrient relocation, cell organization and DNA synthesis. Concomitantly, defense, secondary metabolism and signaling genes were suppressed. Further, real-time PCR validation of a selected set of 20 genes, in three susceptible rice varieties (TN1, Kavya and Suraksha) during the interaction with the respective virulent gall midge biotypes, also revealed variation in gene expression in Kavya as compared to TN1 and Suraksha. Conclusions These studies showed that virulent insects induced the plants to step up metabolism and transport nutrients to their feeding site and suppressed defense responses. But Kavya rice mounted an elevated defense response during early hours of virulent gall midge infestation, which was over-powered later, resulting in host plant susceptibility.
    Rice 01/2012; 5(1). DOI:10.1186/1939-8433-5-8 · 3.92 Impact Factor
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    • "Hierarchical clustering revealed that major enzymes of each pathway displayed a clear coexpression throughout all the conditions studied. Such data mining approaches combined with sequence information allow drawing precise hypotheses on the function of uncharacterized genes (Gachon et al. 2005). Expression of specific genes from three different experiments (Kreps et al. 2002; Seki et al. 2002; Kawaguchi et al. 2004) was compared to understand changes in gene expression in response to water-deficit stress. "
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    ABSTRACT: DNA microarrays became a widely employed tool in functional genomics and global gene expression analysis. They have been intensely used to investigate plant transcriptomes to answer various biological questions involving stress response and tolerance. Identification of stress-related genes via microarrays provides valuable information to improve biotic and abiotic stress tolerance in plants. Since these stresses are the major factors that limit plant growth and production worldwide, in this review recent progresses resulting from gene expression analysis using microarrays under biotic and abiotic stresses are summarized. Moreover microarray technology, manufacturing of arrays, experimental approaches in cDNA and oligonucleotide microarray platforms and data analysis, which are followed by microarray expression profiling studies in plant sciences, are briefly explained.
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